Mouse testis cell sorting according to DNA and mitochondrial changes during spermatogenesis.
Mouse testis cell sorting according to DNA and mitochondrial changes during spermatogenesis.
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根据精子发生过程中 DNA 和线粒体的变化对小鼠睾丸细胞进行分类。
DOI：
10.1002/cyto.990190404
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
R. Julien
中科院分区：
文献类型：
--
作者：
J. Petit;M. Ratinaud;E. Cordelli;M. Spanô;R. Julien
流式细胞术可以测量 DNA 含量和染色质结构的变化，以及生殖细胞从精原细胞成熟到细长精子细胞过程中线粒体的巨大变化。使用 10-N 壬基吖啶橙 (NAO)（一种线粒体内膜染料），可以轻松追踪线粒体重排。用 DNA 荧光探针碘化丙啶 (PI) 染色并通过流式细胞术分析的小鼠睾丸细胞可以根据其倍性水平将其分为五个主要区域，分别对应于细长精子细胞、圆形精子细胞、二倍体、S 期和四倍体细胞。同时使用 PI 和 NAO 证明单倍体、二倍体和四倍体区室中存在具有低和高线粒体含量的细胞。从双变量分析（PI/NAO）中选择11个分选窗口，并通过显微镜观察鉴定相应的细胞。还通过 DNA 含量与细胞直径的两个参数分析来区分细胞。七个不同区域的定义使我们能够确定每个区室中 NAO 或罗丹明 123 (Rh 123) 的摄取量。我们观察到，比率 (Rh 123/NAO) 根据精子发生过程中细胞分化的进展而发生显着变化。
Flow cytometry can measure variations in DNA content and chromatin structure as well as dramatic changes in the mitochondria of germ cells during maturation from spermatogonia to elongated spermatids. Using 10-N nonyl acridine orange (NAO), an inner mitochondrial membrane dye, it is easy to follow mitochondria rearrangements. Mouse testis cells stained with the DNA fluorescent probe propidium iodide (PI) and analyzed by flow cytometry can be discriminated on the basis of their ploidy levels into five main regions corresponding to elongated spermatids, round spermatids, diploid, S-phase, and tetraploid cells. The simultaneous use of PI and NAO demonstrated the presence of cells having low and high mitochondrial content in the haploid, diploid, and tetraploid compartments. Eleven sorting windows were selected from the bivariate analysis (PI/NAO) and the corresponding cells were identified by microscopic observation. Cells were also discriminated by two parameter analysis of DNA content vs. cell diameter. The definition of seven different regions allowed us to determine NAO or rhodamine 123 (Rh 123) uptakes in each compartment. We observed that the ratio (Rh 123/NAO) dramatically changed according to the progression of cell differentiation which occurs during spermatogenesis.
DOI：
10.1007/3-540-29623-9_8674
发表时间：
1986-12
期刊：
The American journal of pathology
影响因子：
--
作者：
J. Robinson;N. Tanphaichitr;A. Bellvé
通讯作者：
J. Robinson;N. Tanphaichitr;A. Bellvé
影响因子：
3.6
作者：
HESS, RA;MILLER, LA;GOLDBERG, E
通讯作者：
GOLDBERG, E
通过根据轴向消光脉冲宽度进行分类，富集指定周期阶段的活淋巴细胞。
DOI：
10.1002/cyto.990030107
发表时间：
1982
期刊：
Cytometry
影响因子：
--
作者：
Monroe,JG;Havran,WL;Cambier,JC
通讯作者：
Cambier,JC