Role of sphingomyelinase in infectious diseases caused by Bacillus cereus.
Role of sphingomyelinase in infectious diseases caused by Bacillus cereus.
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DOI：
10.1371/journal.pone.0038054
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Sakurai J
中科院分区：
文献类型：
--
作者：
Oda M;Hashimoto M;Takahashi M;Ohmae Y;Seike S;Kato R;Fujita A;Tsuge H;Nagahama M;Ochi S;Sasahara T;Hayashi S;Hirai Y;Sakurai J
蜡状芽孢杆菌（B.蜡样芽胞杆菌）是机会性感染中的病原体。在这里，我们表明，蜡状芽孢杆菌鞘磷脂酶（Bc-SMase）是败血症的毒力因子。临床分离株产生大量的Bc-SMase，在体内生长，并导致小鼠死亡，但从土壤中分离的ATCC菌株没有。携带含有Bc-SMase基因的重组质粒的ATCC菌株的一个克隆在体内生长，但携带几乎没有酶活性的E53 A基因的ATCC菌株则不能生长。抗Bc-SMase抗体和Bc-SMase免疫预防了临床分离株引起的死亡，表明Bc-SMase在B引起的疾病中起重要作用。蜡状。在本实验条件下，Bc-SMase处理小鼠巨噬细胞，可明显抑制PGN诱导的H_2O_2产生和吞噬功能，但对TNF-α的释放无影响，对LDH的释放也无明显影响。共聚焦激光显微镜显示，用Bc-SMase处理小鼠巨噬细胞导致形成富含神经酰胺的结构域。光漂白分析表明，Bc-SMase处理的细胞表现出膜流动性降低。结果表明，Bc-SMase是必不可少的SM在膜中的水解，导致吞噬作用的减少。
Bacillus cereus (B. cereus) is a pathogen in opportunistic infections. Here we show that Bacillus cereus sphingomyelinase (Bc-SMase) is a virulence factor for septicemia. Clinical isolates produced large amounts of Bc-SMase, grew in vivo, and caused death among mice, but ATCC strains isolated from soil did not. A transformant of the ATCC strain carrying a recombinant plasmid containing the Bc-SMase gene grew in vivo, but that with the gene for E53A, which has little enzymatic activity, did not. Administration of an anti-Bc-SMase antibody and immunization against Bc-SMase prevented death caused by the clinical isolates, showing that Bc-SMase plays an important role in the diseases caused by B. cereus. Treatment of mouse macrophages with Bc-SMase resulted in a reduction in the generation of H2O2 and phagocytosis of macrophages induced by peptidoglycan (PGN), but no effect on the release of TNF-α and little release of LDH under our experimental conditions. Confocal laser microscopy showed that the treatment of mouse macrophages with Bc-SMase resulted in the formation of ceramide-rich domains. A photobleaching analysis suggested that the cells treated with Bc-SMase exhibited a reduction in membrane fluidity. The results suggest that Bc-SMase is essential for the hydrolysis of SM in membranes, leading to a reduction in phagocytosis.
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影响因子：
2.9
作者：
Montes, L. -Ruth;Lopez, David J.;Sot, Jesus;Bagatolli, Luis A.;Stonehouse, Martin J.;Vasil, Michael L.;Wu, Bill X.;Hannun, Yusuf A.;Goni, Felix M.;Alonso, Alicia
通讯作者：
Alonso, Alicia
影响因子：
6.9
作者：
Dohmae, S.;Okubo, T.;Yamamoto, T.
通讯作者：
Yamamoto, T.
DOI：
10.1016/s1385-299x(03)00016-3
发表时间：
2003-03-01
期刊：
BRAIN RESEARCH PROTOCOLS
影响因子：
--
作者：
Klein, C;Pillot, T;Drouet, B
通讯作者：
Drouet, B
影响因子：
2.8
作者：
Duport, C;Thomassin, S;Schmitt, P
通讯作者：
Schmitt, P
影响因子：
3.1
作者：
Callegan, MC;Cochran, DC;Lereclus, D
通讯作者：
Lereclus, D