PDGF, NT-3 and IGF-2 in combination induced transdifferentiation of muscle-derived stem cells into Schwann cell-like cells.
PDGF, NT-3 and IGF-2 in combination induced transdifferentiation of muscle-derived stem cells into Schwann cell-like cells.
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PDGF、NT-3 和 IGF-2 组合诱导肌源性干细胞转分化为雪旺细胞样细胞
DOI：
10.1371/journal.pone.0073402
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Tang Y;He H;Cheng N;Song Y;Ding W;Zhang Y;Zhang W;Zhang J;Peng H;Jiang H
肌源性干细胞（Muscle-derived stem cells，MDSCs）是一种多能干细胞，具有显著的长期自我更新和再生能力。在这里，我们表明，出生后的MDSCs可以转分化为雪旺细胞样细胞后，联合治疗的三种神经营养因子（PDGF，NT-3和IGF-2）。首先用雪旺细胞条件培养液诱导MDSCs转分化。转分化后的MDSC呈类似于SC的梭形形态。免疫细胞化学和免疫印迹清楚地表明，SC标志物S100、GFAP和p75仅在转分化后才高表达。流式细胞仪检测结果显示，转分化细胞中S100的表达率超过60%，超过1/4的转分化细胞同时表达所有3种SC标志物，表明转分化有效。然后我们测试了条件培养基中的神经营养因子，发现是PDGF、NT-3和IGF-2的组合进行了转分化。我们的研究结果表明，它是可能的，使用特定的神经营养因子转分化MDSCs向许旺细胞样细胞，这可能是治疗上有用的临床应用。
Muscle-derived stem cells (MDSCs) are multipotent stem cells with a remarkable long-term self-renewal and regeneration capacity. Here, we show that postnatal MDSCs could be transdifferentiated into Schwann cell-like cells upon the combined treatment of three neurotrophic factors (PDGF, NT-3 and IGF-2). The transdifferentiation of MDSCs was initially induced by Schwann cell (SC) conditioned medium. MDSCs adopted a spindle-like morphology similar to SCs after the transdifferentiation. Immunocytochemistry and immunoblot showed clearly that the SC markers S100, GFAP and p75 were expressed highly only after the transdifferentiation. Flow cytometry assay showed that the portion of S100 expressed cells was more than 60 percent and over one fourth of the transdifferentiated cells expressed all the three SC markers, indicating an efficient transdifferentiation. We then tested neurotrophic factors in the conditioned medium and found it was PDGF, NT-3 and IGF-2 in combination that conducted the transdifferentiation. Our findings demonstrate that it is possible to use specific neurotrophic factors to transdifferentiate MDSCs into Schwann cell-like cells, which might be therapeutically useful for clinical applications.
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DOI：
10.1083/jcb.200108150
发表时间：
2002-05-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Qu-Petersen Z;Deasy B;Jankowski R;Ikezawa M;Cummins J;Pruchnic R;Mytinger J;Cao B;Gates C;Wernig A;Huard J
通讯作者：
Huard J
DOI：
10.1083/jcb.150.5.1085
发表时间：
2000-09-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lee JY;Qu-Petersen Z;Cao B;Kimura S;Jankowski R;Cummins J;Usas A;Gates C;Robbins P;Wernig A;Huard J
通讯作者：
Huard J
组织干细胞：新工具和功能多样性。
影响因子：
23.9
作者：
Grompe, Markus
通讯作者：
Grompe, Markus
影响因子：
3.3
作者：
Deasy, BM;Gharaibeh, BM;Huard, J
通讯作者：
Huard, J
影响因子：
6.6
作者：
Keilhoff, G;Goihl, A;Wolf, G
通讯作者：
Wolf, G