Serological and Virological Evidence of Crimean-Congo Haemorrhagic Fever Virus Circulation in the Human Population of Borno State, Northeastern Nigeria.
Serological and Virological Evidence of Crimean-Congo Haemorrhagic Fever Virus Circulation in the Human Population of Borno State, Northeastern Nigeria.
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DOI：
10.1371/journal.pntd.0005126
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发表时间：
2016-12
影响因子：
3.8
通讯作者：
Hewson R
中科院分区：
文献类型：
--
作者：
Bukbuk DN;Dowall SD;Lewandowski K;Bosworth A;Baba SS;Varghese A;Watson RJ;Bell A;Atkinson B;Hewson R
尽管对尼日利亚人和牛的克里米亚-刚果出血热病毒 (CCHFV) 抗体血清阳性率进行了几项研究，但针对 IgG 和 IgM 的详细研究尚未见报道。此外，尼日利亚尚未报告人感染 CCHFV 确诊病例。对从博尔诺州四个地方政府区域（Askira/Uba、Damboa、Jere 和 Maiduguri）采集的血清样本（n = 1189）进行了 IgG 和 IgM 抗体的评估。 IgG和IgM的阳性率分别为10.6%和3.5%。此外，还通过 RT-PCR 检测评估了未确诊发热患者 (n = 380) 的血清中是否存在 CCHFV RNA。通过下一代测序 (NGS) 进一步表征一份阳性样本，从而获得完整的 S、M 和 L 片段序列。本文提供了尼日利亚人口持续接触 CCHFV 的证据。基因组分析首次公布了尼日利亚人类 CCHFV 病例及其系统发育背景的证据。克里米亚-刚果出血热（CCHF）是一种急性蜱传人畜共患疾病。病原体 CCHF 病毒 (CCHFV) 在医学上重要的蜱传病毒性疾病中具有最广泛的地理分布，分布在亚洲、中东、非洲的大部分地区以及东南欧的扩大地区。虽然人类感染 CCHFV 的主要途径主要是通过蜱虫叮咬，但它也可以通过体液传播，并且因在医疗机构中引起院内疫情而闻名。虽然自 1970 年以来就在尼日利亚的蜱虫中发现了 CCHFV，但有关人类感染的信息却很少。在本报告中，对人类血清中 CCHFV 反应性抗体的流行情况进行了评估，提供了该病毒在尼日利亚博尔诺州人群中持续传播的证据。此外，在一份样本中检测到病毒 RNA 的存在，从而获得了 CCHFV 的完整序列。这是第一份与尼日利亚人类病例相关的 CCHFV 报告，并且该病毒的完整基因特征已完成。其中的证据支持这样的假设：CCHFV 在尼日利亚流行，应被视为发热患者的病原体。
Despite several studies on the seroprevalence of antibodies against Crimean-Congo Haemorrhagic Fever virus (CCHFV) from humans and cattle in Nigeria, detailed investigation looking at IgG and IgM have not been reported. Additionally, there have been no confirmed cases of human CCHFV infection reported from Nigeria. Samples from sera (n = 1189) collected from four Local Government Areas in Borno State (Askira/Uba, Damboa, Jere and Maiduguri) were assessed for the presence of IgG and IgM antibodies. The positivity rates for IgG and IgM were 10.6% and 3.5%, respectively. Additionally, sera from undiagnosed febrile patients (n = 380) were assessed by RT-PCR assay for the presence of CCHFV RNA. One positive sample was characterised by further by next generation sequencing (NGS) resulting in complete S, M and L segment sequences. This article provides evidence for the continued exposure of the human population of Nigeria to CCHFV. The genomic analysis provides the first published evidence of a human case of CCHFV in Nigeria and its phylogenetic context. Crimean-Congo haemorrhagic fever (CCHF) is an acute tick-borne zoonotic disease. The causative agent, CCHF virus (CCHFV), has the most extensive geographical distribution of the medically important tick-borne viral diseases with a distribution over much of Asia, the Middle East, Africa and expanding areas of south-eastern Europe. Whilst the main route of human infection with CCHFV is predominantly through tick bite, it can also be spread via bodily fluids and it has a reputation for causing nosocomial outbreaks in healthcare settings. Whilst CCHFV has been identified in ticks from Nigeria since 1970, there is scarce information on human infection. Within this report, the prevalence of CCHFV-reactive antibodies has been assessed in human sera providing evidence of continued circulation of the virus in the human population of Borno state, Nigeria. Additionally, in one sample the presence of viral RNA was detected which allowed a full sequence of the CCHFV to be obtained. This is the first report of CCHFV being associated in a human case from Nigeria and the full genetic characterisation of the virus being completed. The evidence within supports the hypothesis that CCHFV is endemic in Nigeria and should be considered as an aetiological agent in febrile patients.
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影响因子：
3.8
作者：
Fajs L;Jakupi X;Ahmeti S;Humolli I;Dedushaj I;Avšič-Županc T
通讯作者：
Avšič-Županc T
影响因子：
3.1
作者：
Dowall, S. D.;Richards, K. S.;Hewson, R.
通讯作者：
Hewson, R.
DOI：
10.4269/ajtmh.1970.19.670
发表时间：
1970-01-01
影响因子：
3.3
作者：
FRAME, JD;BALDWIN, JM;TROUP, JM
通讯作者：
TROUP, JM
DOI：
10.4269/ajtmh.2009.09-0051
发表时间：
2009-10-01
影响因子：
3.3
作者：
Mardani, Masoud;Keshtkar-Jahromi, Maryam;Adibi, Peyman
通讯作者：
Adibi, Peyman
影响因子：
8.4
作者：
Pshenichnaya, Natalia Yurievna;Nenadskaya, Svetlana Alexeevna
通讯作者：
Nenadskaya, Svetlana Alexeevna