Molecular epidemiology of Crimean-Congo hemorrhagic fever virus in Kosovo.
Molecular epidemiology of Crimean-Congo hemorrhagic fever virus in Kosovo.
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DOI：
10.1371/journal.pntd.0002647
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发表时间：
2014
影响因子：
3.8
通讯作者：
Avšič-Županc T
中科院分区：
文献类型：
--
作者：
Fajs L;Jakupi X;Ahmeti S;Humolli I;Dedushaj I;Avšič-Županc T
克里米亚-刚果出血热病毒（CCHFV）是一种人畜共患病原体，可引起严重的危及生命的疾病，病死率为10- 50%。它是世界上传播最广的蜱传病毒，在非洲、亚洲和东欧都有病例报告。CCHFV是一种遗传多样性病毒。其遗传多样性往往与其地理来源有关。CCHFV的遗传变异性在少数流行地区确定，但有限的数据是在科索沃。此外，关于流行区CCHFV的时空遗传变化的信息很少。科索沃是CCHFV的重要流行区。每年都有病例报告，病死率明显高于邻近地区。在这项研究中，我们想检查直接从1991年至2013年在科索沃住院的CCHF确诊患者中获得的CCHFV的遗传变异性。我们测定了89例CCHFV患者的部分S片段核苷酸序列。我们的研究结果表明，几种病毒变种存在于科索沃和遗传多样性是高的研究领域。我们还表明，变异大多是均匀分布在整个科索沃和有限的进化变化发生在22年。我们的研究结果还表明存在一个新的独特的血统内的欧洲CCHF系统发育支。我们的研究提供了最大数量的CCHFV核苷酸序列的病人在22年的跨度在一个流行区。克里米亚-刚果出血热（CCHF）是一种急性蜱传疾病，病死率为10- 30%。它是世界上地理分布最广的蜱传疾病。近年来，几个国家的发病率有所上升，主要是在巴尔干半岛的国家。这种疾病在科索沃也是地方病。由于CCHF病毒的遗传多样性非常大，我们的目标是确定科索沃22年内病毒的遗传变异性。我们获得了最大数量的患者来源的核苷酸序列，并发现了很大的遗传变异性，在22年期间或多或少保持稳定。我们的研究结果还表明，病毒种群的显着变化发生在不同的年份。我们表明，生态因素，如温度可能在病毒种群的组成中发挥作用。
Crimean-Congo hemorrhagic fever virus (CCHFV) is a zoonotic agent that causes severe, life-threatening disease, with a case fatality rate of 10–50%. It is the most widespread tick-borne virus in the world, with cases reported in Africa, Asia and Eastern Europe. CCHFV is a genetically diverse virus. Its genetic diversity is often correlated to its geographical origin. Genetic variability of CCHFV was determined within few endemic areas, however limited data is available for Kosovo. Furthermore, there is little information about the spatiotemporal genetic changes of CCHFV in endemic areas. Kosovo is an important endemic area for CCHFV. Cases were reported each year and the case-fatality rate is significantly higher compared to nearby regions. In this study, we wanted to examine the genetic variability of CCHFV obtained directly from CCHF-confirmed patients, hospitalized in Kosovo from 1991 to 2013. We sequenced partial S segment CCHFV nucleotide sequences from 89 patients. Our results show that several viral variants are present in Kosovo and that the genetic diversity is high in relation to the studied area. We also show that variants are mostly uniformly distributed throughout Kosovo and that limited evolutionary changes have occurred in 22 years. Our results also suggest the presence of a new distinct lineage within the European CCHF phylogenetic clade. Our study provide the largest number of CCHFV nucleotide sequences from patients in 22 year span in one endemic area. Crimean-Congo hemorrhagic fever (CCHF) is an acute, tick-borne disease with a case fatality rate of 10–30%. It is geographically the most widespread tick-borne disease in the world. In recent years there has been an increase of the disease incidence in several countries, mainly in the countries of the Balkan. The disease is also endemic in Kosovo. Since CCHF virus is very genetically diverse we aimed to determine the genetic variability of the virus in Kosovo in the span of 22 years. We obtained the largest number of patient derived nucleotide sequences and found great genetic variability which has been more or less stable during the 22 year period. Our results also suggest that significant changes in viral population occur in different years. We show that ecological factors such as temperature could play a role in the composition of the viral population.
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影响因子：
8.4
作者：
Elevli, Murat;Ozkul, Ayse Ayaz;Duru, Nilgun Selcuk
通讯作者：
Duru, Nilgun Selcuk
影响因子：
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通讯作者：
Doms, Robert W.
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作者：
Ozkaya, Etem;Dincer, Ender;Ozkul, Aykut
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Ozkul, Aykut
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12.7
作者：
Papa, A;Papadimitriou, E;Antoniadis, A
通讯作者：
Antoniadis, A