MicroRNAs of two medically important mosquito species: Aedes aegypti and Anopheles stephensi.
MicroRNAs of two medically important mosquito species: Aedes aegypti and Anopheles stephensi.
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DOI：
10.1111/imb.12152
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发表时间：
2015-04
影响因子：
2.6
通讯作者：
Tu Z
中科院分区：
文献类型：
--
作者：
Hu W;Criscione F;Liang S;Tu Z
MicroRNAs (miRNAs) are endogenous, single-stranded small RNAs that have important regulatory functions at the post-transcriptional level. Here, we characterize miRNAs in two divergent mosquito species, Aedes aegypti and Anopheles stephensi, through deep sequencing of small RNAs spanning all developmental stages. We discovered eight novel miRNAs in Ae. aegypti and 20 novel miRNAs in An. stephensi, which enabled the first systematic analysis of miRNA evolution in mosquitos. We traced the phylogenetic history of all miRNAs in both species and report a rate of 0.055–0.13 miRNA net gain per million years. Most novel miRNAs originate de novo. Duplications that produced miRNA clusters and families are more common in Ae. aegypti than in An. stephensi. We also identified arm-switch as a source of new miRNAs. Expression profile analysis identified mosquito-specific miRNAs that showed strong stage-specific expression in one or both lineages. For example, the aae-miR-2941/2946 family represents the most abundant maternally-deposited and zygotically transcribed miRNAs in Ae. aegypti. miR-2943 is a highly expressed zygotic miRNA in both Ae. aegypti and An. stephensi. Such information provides the basis to study the function of these miRNAs in biology common to all mosquitos or unique to one particular lineage.
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影响因子：
3.7
作者：
Gu J;Hu W;Wu J;Zheng P;Chen M;James AA;Chen X;Tu Z
通讯作者：
Tu Z
影响因子：
4.4
作者：
Biryukova, Inna;Ye, Tao;Levashina, Elena
通讯作者：
Levashina, Elena
影响因子：
12.3
作者：
Jiang X;Peery A;Hall AB;Sharma A;Chen XG;Waterhouse RM;Komissarov A;Riehle MM;Shouche Y;Sharakhova MV;Lawson D;Pakpour N;Arensburger P;Davidson VL;Eiglmeier K;Emrich S;George P;Kennedy RC;Mane SP;Maslen G;Oringanje C;Qi Y;Settlage R;Tojo M;Tubio JM;Unger MF;Wang B;Vernick KD;Ribeiro JM;James AA;Michel K;Riehle MA;Luckhart S;Sharakhov IV;Tu Z
通讯作者：
Tu Z
影响因子：
3
作者：
Guerra-Assunção JA;Enright AJ
通讯作者：
Enright AJ
影响因子：
3.4
作者：
Biedler JK;Tu Z
通讯作者：
Tu Z