Integrated analysis of small RNA, transcriptome and degradome sequencing reveals that micro-RNAs regulate anther development in CMS cotton
Integrated analysis of small RNA, transcriptome and degradome sequencing reveals that micro-RNAs regulate anther development in CMS cotton
复制标题
小RNA、转录组和降解组测序的综合分析揭示了微小RNA调控CMS棉花花药发育
DOI：
10.1016/j.indcrop.2021.114422
复制
发表时间：
2022-02
影响因子：
5.9
通讯作者：
Chaozhu Xing
中科院分区：
文献类型：
--
作者：
Yongqi Li;Taili Nie;Meng Zhang;Xuexian Zhang;Kashif Shahzad;Liping Guo;Tingxiang Qi;Huini Tang;Hailin Wang;Xiuqin Qiao;Juanjuan Feng;Zhongxu Lin;Jianyong Wu;Chaozhu Xing
细胞质雄性不育（CMS）是棉花杂种优势利用的重要表型性状。微rna （miRNAs）在植物花粉发育中起着至关重要的作用。为了阐明mirna靶基因对在棉花不育系甘绵15a及其保持系甘绵15b中的作用，本研究对甘绵15b和甘绵15a进行了花粉败育阶段的小RNA （sRNA）、转录组和降解组测序分析。通过高通量测序共鉴定出339个已知mirna和961个保守mirna，其中124个在ganmian15A和ganmian15B之间存在差异表达。通过综合分析miRNA、转录组和降解组测序，我们在花粉败育期鉴定出11对miRNA /靶基因。其中，首次发现9个与棉花雄性不育发生相关的mirna（4个新发现，5个已知）。miRna-38和miRna-23的靶基因与花粉期细胞壁发育有关。它们可能通过延缓绒毡层细胞的降解而在CMS棉花中发挥重要作用。本研究结果为CMS发生的遗传控制提供了新的见解，为今后棉花不育新品系的选育提供了依据。
Cytoplasmic male sterility (CMS) is an important phenotypic trait for heterosis utilization in cotton (Gosspium hirsutumL.). Micro-RNAs (miRNAs) play crucial roles in the pollen development of plants. To elucidate the roles of miRNA-target gene pairs in the CMS cotton line ganmian15A and its maintainer line ganmian15B, an integrated small RNA (sRNA), transcriptome, and degradome sequencing analysis of these lines at the pollen abortion stage was performed in the present research. A total of 339 known miRNAs and 961 conserved miRNAs were identified through high-throughput sequencing, among which 124 were differentially expressed between ganmian15A and ganmian15B. By comprehensive analysis of miRNA, transcriptome, and degradome sequencing, we identified 11 pairs of miRNAs/target genes at the pollen abortion stage. Among them, 9 miRNAs (4 novel and 5 known) were found for the first time to be associated with the occurrence of male sterility in cotton. The target genes of miRna-38 and miRna-23 were linked to cell wall development at the pollen stage. They may play an imperative role in CMS cotton by delaying the degradation of tapetal cells. Our findings revealed new insights into the genetic control of CMS occurrence, which will support the breeding of new sterile lines of cotton in the future.
登录
查看更多内容
影响因子：
5.6
作者：
Zhang W;Xie Y;Xu L;Wang Y;Zhu X;Wang R;Zhang Y;Muleke EM;Liu L
通讯作者：
Liu L
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
影响因子：
4.4
作者：
Shamimuzzaman M;Vodkin L
通讯作者：
Vodkin L
影响因子：
2.3
作者：
D. Weaver;J. B. Weaver
通讯作者：
D. Weaver;J. B. Weaver
影响因子：
7.4
作者：
Min, Ling;Li, Yaoyao;Zhang, Xianlong
通讯作者：
Zhang, Xianlong