The ratio 1660/1690 cm(-1) measured by infrared microspectroscopy is not specific of enzymatic collagen cross-links in bone tissue.
The ratio 1660/1690 cm(-1) measured by infrared microspectroscopy is not specific of enzymatic collagen cross-links in bone tissue.
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DOI：
10.1371/journal.pone.0028736
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Boivin G
中科院分区：
文献类型：
--
作者：
Farlay D;Duclos ME;Gineyts E;Bertholon C;Viguet-Carrin S;Nallala J;Sockalingum GD;Bertrand D;Roger T;Hartmann DJ;Chapurlat R;Boivin G
在绝经后骨质疏松症中，酶交联（ECL）受损，部分导致骨生物力学性能下降。目前，通过高效液相色谱法（HPLC）的生化方法用于测量ECL。傅里叶变换红外成像（FTIRI）提出了另一种方法，使用酰胺I谱带中1660/1690 cm-1的面积比来测量成熟PYD/未成熟DHLNL交联比。然而，在骨中，酰胺I带组成复杂（胶原、非胶原蛋白、水振动），并且FTIRI的1660/1690 cm−1从未与HPLC的PYD/DHLNL直接相关。采用了一项使用laectic大鼠的研究设计，其特征在于由于赖氨酰氧化酶的抑制导致ECL形成减少，以通过FTIR显微光谱法测定骨组织中1660/1690 cm−1的变化，并与HPLC定量的ECL进行比较。通过高效液相色谱法定量测定对照组和蹄铁下炎大鼠皮质骨中ECL的实际含量。与对照组相比，乳酸菌组的吡啶啉含量降低了78%。1660/1690 cm-1面积比在中心骨内比在内部骨内增加，这也与矿物成熟度和矿化指数的增加有关。然而，在对照组和lavitic大鼠之间没有发现1660/1690 cm-1比值的差异。对多光谱红外图像进行的主成分分析证实了这些结果。在牛骨中，其中PYD通过UV光解被物理破坏，PYD/DHLNL（通过HPLC测量）强烈降低，而1660/1690 cm−1未被改变。总之，1660/1690 cm−1与PYD/DHLNL比值无关，但随着骨矿物质年龄的增加而增加，这表明该比值的改变可能主要是由于与矿化过程相关的胶原蛋白二级结构的改变。
In postmenopausal osteoporosis, an impairment in enzymatic cross-links (ECL) occurs, leading in part to a decline in bone biomechanical properties. Biochemical methods by high performance liquid chromatography (HPLC) are currently used to measure ECL. Another method has been proposed, by Fourier Transform InfraRed Imaging (FTIRI), to measure a mature PYD/immature DHLNL cross-links ratio, using the 1660/1690 cm−1 area ratio in the amide I band. However, in bone, the amide I band composition is complex (collagens, non-collagenous proteins, water vibrations) and the 1660/1690 cm−1 by FTIRI has never been directly correlated with the PYD/DHLNL by HPLC. A study design using lathyritic rats, characterized by a decrease in the formation of ECL due to the inhibition of lysyl oxidase, was used in order to determine the evolution of 1660/1690 cm−1 by FTIR Microspectroscopy in bone tissue and compare to the ECL quantified by HPLC. The actual amount of ECL was quantified by HPLC on cortical bone from control and lathyritic rats. The lathyritic group exhibited a decrease of 78% of pyridinoline content compared to the control group. The 1660/1690 cm−1 area ratio was increased within center bone compared to inner bone, and this was also correlated with an increase in both mineral maturity and mineralization index. However, no difference in the 1660/1690 cm−1 ratio was found between control and lathyritic rats. Those results were confirmed by principal component analysis performed on multispectral infrared images. In bovine bone, in which PYD was physically destructed by UV-photolysis, the PYD/DHLNL (measured by HPLC) was strongly decreased, whereas the 1660/1690 cm−1 was unmodified. In conclusion, the 1660/1690 cm−1 is not related to the PYD/DHLNL ratio, but increased with age of bone mineral, suggesting that a modification of this ratio could be mainly due to a modification of the collagen secondary structure related to the mineralization process.
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DOI：
10.1080/10255840701479248
发表时间：
2007-08-01
影响因子：
1.6
作者：
Le Quang, T.;Maitre, P.;Viguier, E.
通讯作者：
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影响因子：
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作者：
KUNDEL, HL
通讯作者：
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影响因子：
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作者：
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影响因子：
2.9
作者：
LAZAREV, YA;GRISHKOVSKY, BA;KHROMOVA, TB
通讯作者：
KHROMOVA, TB
影响因子：
2.9
作者：
Dowd, TL;Rosen, JF;Gundberg, CM
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Gundberg, CM