Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang.
Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang.
复制标题
桑黄桑黄赖氨酸丙二醛基因组的系统分析
DOI：
10.1186/s12864-021-08120-0
复制
发表时间：
2021-11-19
期刊：
影响因子：
4.4
通讯作者：
Tian X
中科院分区：
文献类型：
--
作者：
Wang T;Wang G;Zhang G;Hou R;Zhou L;Tian X
背景
桑黄孢菌（Sanghuangporus sanghuang）是一种与桑葚相关的著名传统药用菌。尽管这种蘑菇的特性已经知道了很多年，但这种药用蘑菇中生物活性化合物生物合成的调控机制仍然不清楚。赖氨酸丙二酰化是一种翻译后修饰，在细胞代谢的各个方面具有许多关键功能。然而，目前我们还不知道它在S。桑黄。在这项研究中，赖氨酸丙二酸组在S。桑黄因此被执行。
结果
总共有714个丙二酰修饰位点与255种不同的蛋白质相匹配。分析表明，丙二酰修饰涉及广泛的细胞功能，并显示出独特的亚细胞定位。生物信息学分析表明，丙二酰化蛋白参与了不同的代谢途径，包括乙醛酸和二羧酸代谢、糖酵解/异源生成和三羧酸（TCA）循环。其中，共有26种与三萜和多糖生物合成相关的酶被丙二酰化，表明赖氨酸丙二酰化在S.桑黄。
结论
这些结果表明，丙二酰化与许多代谢途径，特别是生物活性化合物三萜和多糖的代谢。本文首次对S.为进一步研究赖氨酸丙二酰化的生理功能提供了重要数据。桑黄等药用菌。
Background
Sanghuangporus sanghuang is a well-known traditional medicinal mushroom associated with mulberry. Despite the properties of this mushroom being known for many years, the regulatory mechanisms of bioactive compound biosynthesis in this medicinal mushroom are still unclear. Lysine malonylation is a posttranslational modification that has many critical functions in various aspects of cell metabolism. However, at present we do not know its role in S. sanghuang. In this study, a global investigation of the lysine malonylome in S. sanghuang was therefore carried out.
Results
In total, 714 malonyl modification sites were matched to 255 different proteins. The analysis indicated that malonyl modifications were involved in a wide range of cellular functions and displayed a distinct subcellular localization. Bioinformatics analysis indicated that malonylated proteins were engaged in different metabolic pathways, including glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and the tricarboxylic acid (TCA) cycle. Notably, a total of 26 enzymes related to triterpene and polysaccharide biosynthesis were found to be malonylated, indicating an indispensable role of lysine malonylation in bioactive compound biosynthesis in S. sanghuang.
Conclusions
These findings suggest that malonylation is associated with many metabolic pathways, particularly the metabolism of the bioactive compounds triterpene and polysaccharide. This paper represents the first comprehensive survey of malonylation in S. sanghuang and provides important data for further study on the physiological function of lysine malonylation in S. sanghuang and other medicinal mushrooms.
登录
查看更多内容
影响因子：
3.8
作者：
Bradley NL;Wiseman SM
通讯作者：
Wiseman SM
影响因子：
4.4
作者：
Liu J;Wang G;Lin Q;Liang W;Gao Z;Mu P;Li G;Song L
通讯作者：
Song L
DOI：
10.1093/bioinformatics/bts251
发表时间：
2012-07-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Jiao X;Sherman BT;Huang da W;Stephens R;Baseler MW;Lane HC;Lempicki RA
通讯作者：
Lempicki RA
影响因子：
3.7
作者：
Gao, Yuan;Li, Shipeng;Dang, Ruihua
通讯作者：
Dang, Ruihua
影响因子：
9.3
作者：
Cao, Liu;Gurevich, Alexey;Mohimani, Hosein
通讯作者：
Mohimani, Hosein