The Phytophthora sojae avirulence locus Avr3c encodes a multi-copy RXLR effector with sequence polymorphisms among pathogen strains.
The Phytophthora sojae avirulence locus Avr3c encodes a multi-copy RXLR effector with sequence polymorphisms among pathogen strains.
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DOI：
10.1371/journal.pone.0005556
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Gijzen M
中科院分区：
文献类型：
--
作者：
Dong S;Qutob D;Tedman-Jones J;Kuflu K;Wang Y;Tyler BM;Gijzen M
大豆根腐病是由大豆疫霉（Phytophthora sojae）卵菌引起的大豆根腐病。大豆疫霉的无毒基因（Avr）控制小种-品种亲和性。在这项研究中，我们鉴定了大豆疫霉菌Avr 3c基因，并表明它编码预测的220个氨基酸的RXLR效应蛋白。序列和转录数据进行了比较预测的RXLR效应发生在附近的Avr 4/6，Avr 3c和Avr 4/6的遗传连锁先前提出。在F2群体中进行DNA标记的定位以确定所选择的RXLR效应基因是否与Avr 3c表型共分离。结果指出一个RXLR候选基因可能编码Avr 3c。这通过用Rps 3c对大豆植物进行共轰击测定来测试所选基因来验证，从而证明了Avr 3c的功能性并确认了Avr 3c的身份。Avr 3c基因与其他8个预测基因一起是33.7 kb重复片段的一部分。该片段的三个几乎相同的拷贝出现在串联阵列中。在大豆疫霉菌株P6497中，Avr 3c的两个相同拷贝出现在重复区段内，而该RXLR效应子的第三个拷贝在序列上有分歧。Avr 3c基因在所有检测的大豆疫霉菌株中在感染的早期阶段表达。在大豆疫霉的其他菌株中鉴定出氨基酸序列不同的Avr 3c的毒力等位基因。通过突变和随后的Avr 3c两个拷贝之间的序列交换获得毒力。结果说明了片段复制和RXLR效应子进化在大豆疫霉菌和大豆相互作用中控制小种-品种相容性的重要性。
Root and stem rot disease of soybean is caused by the oomycete Phytophthora sojae. The avirulence (Avr) genes of P. sojae control race-cultivar compatibility. In this study, we identify the P. sojae Avr3c gene and show that it encodes a predicted RXLR effector protein of 220 amino acids. Sequence and transcriptional data were compared for predicted RXLR effectors occurring in the vicinity of Avr4/6, as genetic linkage of Avr3c and Avr4/6 was previously suggested. Mapping of DNA markers in a F2 population was performed to determine whether selected RXLR effector genes co-segregate with the Avr3c phenotype. The results pointed to one RXLR candidate gene as likely to encode Avr3c. This was verified by testing selected genes by a co-bombardment assay on soybean plants with Rps3c, thus demonstrating functionality and confirming the identity of Avr3c. The Avr3c gene together with eight other predicted genes are part of a repetitive segment of 33.7 kb. Three near-identical copies of this segment occur in a tandem array. In P. sojae strain P6497, two identical copies of Avr3c occur within the repeated segments whereas the third copy of this RXLR effector has diverged in sequence. The Avr3c gene is expressed during the early stages of infection in all P. sojae strains examined. Virulent alleles of Avr3c that differ in amino acid sequence were identified in other strains of P. sojae. Gain of virulence was acquired through mutation and subsequent sequence exchanges between the two copies of Avr3c. The results illustrate the importance of segmental duplications and RXLR effector evolution in the control of race-cultivar compatibility in the P. sojae and soybean interaction.
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DOI：
10.1073/pnas.0709303105
发表时间：
2008-03-25
影响因子：
11.1
作者：
Jiang, Rays H. Y.;Tripathy, Sucheta;Tyler, Brett M.
通讯作者：
Tyler, Brett M.
影响因子：
11.6
作者：
Dou, Daolong;Kale, Shiv D.;Tyler, Brett M.
通讯作者：
Tyler, Brett M.
影响因子：
3.7
作者：
Qutob D;Tedman-Jones J;Dong S;Kuflu K;Pham H;Wang Y;Dou D;Kale SD;Arredondo FD;Tyler BM;Gijzen M
通讯作者：
Gijzen M
影响因子：
56.9
作者：
Allen, RL;Bittner-Eddy, PD;Beynon, JL
通讯作者：
Beynon, JL
影响因子：
7
作者：
Jiang, Rays H. Y.;Weide, Rob;Govers, Francine
通讯作者：
Govers, Francine