Increased peripheral and local soluble FGL2 in the recovery of renal ischemia reperfusion injury in a porcine kidney auto-transplantation model.
Increased peripheral and local soluble FGL2 in the recovery of renal ischemia reperfusion injury in a porcine kidney auto-transplantation model.
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猪肾自体移植模型肾缺血再灌注损伤恢复过程中外周和局部可溶性 FGL2 的增加
DOI：
10.1186/1479-5876-12-53
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发表时间：
2014-02-23
影响因子：
7.4
通讯作者：
Zhu T
中科院分区：
文献类型：
--
作者：
Zhao Z;Yang C;Li L;Zhao T;Wang L;Rong R;Yang B;Xu M;Zhu T
研究背景调节性T细胞（Regulatory T cells，Treg）通过抑制天然免疫对肾脏缺血再灌注损伤具有保护作用，但其机制尚未完全阐明。可溶性纤维蛋白原样蛋白2（sFGL 2）是Treg的一种新型效应子，可影响细胞凋亡和炎症反应。本研究探讨了sFGL 2在猪肾自体移植模型中肾IR损伤中的作用。材料和方法从小型猪中取出左肾，用威斯康星州大学溶液注入肾动脉，夹闭肾动脉和静脉，冷藏24 h。右肾切除后，将左肾自体移植到右肾2周。分别于移植后第2、5、7、10、14天处死3头猪。收集的肾组织和每日的血液样本被储存作进一步analysis.ResultsBoth血清肌酐和血尿素氮最大化，在第2至5天，随后逐渐恢复超过2周。肾脏组织学损害、髓过氧化物酶阳性细胞和细胞凋亡以及循环TNF-α和IFN-γ均呈相似模式。血清sFGL 2呈波动性升高，第10天达到峰值。sFGL 2及其受体FcγRIIB、Foxp 3和IL-10在移植后第5 ~ 10天表达显著增加。结论在2周猪自体移植模型中，sFGL 2及其受体FcγRIIB在移植后恢复过程中表达增加，提示sFGL 2可能是一种潜在的肾脏保护介质，参与了移植肾的自我修复和重建。
BackgroundRegulatory T cells (Treg) protect kidney against ischemia reperfusion (IR) injury via suppressing innate immunity, but the mechanism has not been fully clarified. Soluble fibrinogen-like protein 2 (sFGL2), a novel effector of Treg, may affect apoptosis and inflammation. This study investigated the role of sFGL2 in renal IR injury in a porcine kidney auto-transplantation model.Materials and methodsThe left kidney was retrieved from mini pigs and infused by University of Wisconsin solution into the renal artery with the renal artery and vein clamped for 24-h cold storage. After the right nephrectomy, the left kidney was auto-transplanted into the right for 2 weeks. 3 pigs were sacrificed at day 2, 5, 7, 10 and 14 post-transplantation respectively. Collected renal tissues and daily blood samples were stored for further analyses.ResultsBoth serum creatinine and blood urea nitrogen were maximized during day 2 to 5 and followed by a gradual recovery over 2 weeks. The similar pattern were showed in histological damage, myeloperoxidase + cells and apoptosis in the kidney, as well as circulating TNF-α and IFN-γ. Serum sFGL2 presented a fluctuating increase and reached a peak at day 10. The expression of sFGL2 and its receptor FcγRIIB as well as Foxp3 and IL-10 in the kidney was notably increased from day 5 to 10.ConclusionThe increased sFGL2 together with FcγRIIB during renal recovery after IR injury suggested that sFGL2 might be a potential renoprotective mediator involved in the renal self-repairing and remodeling in this 2-week porcine auto-transplantation model.
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DOI：
10.18632/aging.100459
发表时间：
2012-05
期刊：
Aging
影响因子：
--
作者：
Favaloro B;Allocati N;Graziano V;Di Ilio C;De Laurenzi V
通讯作者：
De Laurenzi V
DOI：
10.1186/1756-9966-30-87
发表时间：
2011-09-26
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Wong RS
通讯作者：
Wong RS
DOI：
10.1007/978-94-007-2869-1_7
发表时间：
2012-01-01
期刊：
ADVANCES IN MITOCHONDRIAL MEDICINE
影响因子：
--
作者：
Estaquier, Jerome;Vallette, Francois;Mignotte, Bernard
通讯作者：
Mignotte, Bernard
影响因子：
0.9
作者：
Du, C;Guan, Q;Jevnikar, AM
通讯作者：
Jevnikar, AM
影响因子：
6.2
作者：
Guan, Qiunong;Nguan, Christopher Y. C.;Du, Caigan
通讯作者：
Du, Caigan