CFHTF2 Is Needed for Vegetative Growth, Conidial Morphogenesis and the Osmotic Stress Response in the Tea Plant Anthracnose (Colletotrichum fructicola).
CFHTF2 Is Needed for Vegetative Growth, Conidial Morphogenesis and the Osmotic Stress Response in the Tea Plant Anthracnose (Colletotrichum fructicola).
复制标题
CFHTF2是营养生长，分生孢子形态发生和植物炭疽病（Colletotrichum Fructicola）的渗透应激反应所必需的。
作者：
茶叶是世界范围内重要的经济作物，其营养价值决定了其较高的经济效益。茶叶炭疽病是茶树的常见病害，严重影响食用安全和产量，对茶产业的可持续发展影响深远。本研究对茶炭疽病同源框转录调节因子HTF 2的敲除菌株和互补菌株进行了表型分析和致病性分析，并通过RNA-seq探讨了这些菌株的致病机制。对稻瘟病菌MoHox 1基因序列进行了索引，并在炭疽病基因组中搜索了CfHTF 2。进化分析最近报道了HTF 2对C. fructicola和C. Higginsianum。CfHTF_2的缺失降低了C.削弱了其对逆境的抵抗力和致病力。对野生型N425和CfHTF 2缺失突变体进行转录组测序，共获得3144个差异表达基因（DEG），其中上调基因1594个，下调基因1550个。GO和KEGG富集分析DEG主要集中在信号通路，如次生代谢产物的生物合成。本研究为进一步研究茶树炭疽病的致病机理奠定了基础，为分析CfHTF 2的致病分子机制提供了分子基础。
Tea is an important cash crop worldwide, and its nutritional value has led to its high economic benefits. Tea anthracnose is a common disease of tea plants that seriously affects food safety and yield and has a far-reaching impact on the sustainable development of the tea industry. In this study, phenotypic analysis and pathogenicity analysis were performed on knockout and complement strains of HTF2—the transcriptional regulator of tea anthracnose homeobox—and the pathogenic mechanism of these strains was explored via RNA-seq. The MoHox1 gene sequence of the rice blast fungus was indexed, and the anthracnose genome was searched for CfHTF2. Evolutionary analysis recently reported the affinity of HTF2 for C. fructicola and C. higginsianum. The loss of CfHTF2 slowed the vegetative growth and spore-producing capacity of C. fructicola and weakened its resistance and pathogenesis to adverse conditions. The transcriptome sequencing of wild-type N425 and CfHTF2 deletion mutants was performed, and a total of 3144 differentially expressed genes (DEGs) were obtained, 1594 of which were upregulated and 1550 of which were downregulated. GO and KEGG enrichment analyses of DEGs mainly focused on signaling pathways such as the biosynthesis of secondary metabolites. In conclusion, this study lays a foundation for further study of the pathogenic mechanism of tea anthracnose and provides a molecular basis for the analysis of the pathogenic molecular mechanism of CfHTF2.
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影响因子：
16.5
作者：
Cannon PF;Damm U;Johnston PR;Weir BS
通讯作者：
Weir BS
DOI：
10.3390/jof9060623
发表时间：
2023-05-28
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.5
作者：
Kim S;Park SY;Kim KS;Rho HS;Chi MH;Choi J;Park J;Kong S;Park J;Goh J;Lee YH
通讯作者：
Lee YH
影响因子：
3.3
作者：
Ostapenko, Denis;Solomon, Mark J.
通讯作者：
Solomon, Mark J.
影响因子：
11.4
作者：
GARBER, RL;KUROIWA, A;GEHRING, WJ
通讯作者：
GEHRING, WJ