Chronic ethanol increases liver plasma membrane fluidity.
Chronic ethanol increases liver plasma membrane fluidity.
复制标题
慢性乙醇会增加肝脏质膜的流动性。
作者：
M. Polokoff;Thomas J. Simon;R. Adron Harris;Francis R. Simin;Mieko Iwahashi
Purified plasma membrane fractions of cultured well-differentiated Reuber H35 hepatoma cells were studied after growth in the presence or absence of ethanol. Growth of cells in the presence of ethanol significantly increased plasma membrane 5'-nucleotidase activity but did not influence sodium-potassium adenosinetriphosphatase activity. Fluorescence polarization of lipophilic probes was used to study membrane lipid structure. Steady-state polarization of diphenylhexatriene (DPH), a probe of the hydrophobic core, was significantly lower in plasma membranes from cells grown in 80 mM ethanol for 3 weeks, compared to controls. Decreased polarization of DPH in plasma membranes was observed after 3-weeks growth of cells in as little as 1 mM ethanol. A 1-h exposure to 80 mM ethanol had no effect. Altered DPH polarization was due to a decrease in the order parameter of the probe. The rotational correlation time of the probe was virtually unchanged. Chronic ethanol treatment of cells did not alter the polarization of the membrane surface probe trimethylammoniodiphenylhexatriene. Plasma membranes from cells grown in 80 mM ethanol had decreased contents of both phospholipid and unesterified cholesterol, but the cholesterol to phospholipid ratio was unchanged. The percentages of sphingomyelin and phosphatidylserine in plasma membrane phospholipids were significantly decreased after ethanol treatment, while the phosphatidylcholine/sphingomyelin ratio was increased by 42%. Vesicles prepared from total plasma membrane lipids of ethanol-treated cells, as well as vesicles prepared from polar lipids alone, showed the same alterations in DPH polarization as did plasma membranes. The importance of ethanol metabolism in the observed plasma membrane changes was demonstrated in two ways.(ABSTRACT TRUNCATED AT 250 WORDS)
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长期服用乙醇会诱导豚鼠突触质膜中磷脂酰丝氨酸的增加。
DOI：
10.1016/0006-291x(83)90460-6
发表时间：
1983
影响因子：
3.1
作者：
Sun,GY;Sun,AY
通讯作者：
Sun,AY
乙醇耐受依赖性小鼠脑膜的物理特性和脂质组成。
影响因子：
3.6
作者：
Harris,RA;Baxter,DM;Mitchell,MA;Hitzemann,RJ
通讯作者：
Hitzemann,RJ
慢性酗酒者的耐受性和交叉耐受性：乙醇和其他药物的膜结合减少。
DOI：
10.1126/science.6264608
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rottenberg,H;Waring,A;Rubin,E
通讯作者：
Rubin,E
慢性乙醇消耗对肝线粒体膜温度依赖性物理特性的影响。
影响因子：
3.9
作者：
Waring,AJ;Rottenberg,H;Ohnishi,T;Rubin,E
通讯作者：
Rubin,E
长期摄入乙醇会增加肝微粒体中钙的吸收和对乙醇分子紊乱的抵抗力。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ponnappa,BC;Waring,AJ;Hoek,JB;Rottenberg,H;Rubin,E
通讯作者：
Rubin,E