Echinococcus granulosus sensu stricto: silencing of thioredoxin peroxidase impairs the differentiation of protoscoleces into metacestodes.
Echinococcus granulosus sensu stricto: silencing of thioredoxin peroxidase impairs the differentiation of protoscoleces into metacestodes.
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严格意义上的细粒棘球绦虫：硫氧还蛋白过氧化物酶的沉默会损害原头节向后头节的分化
DOI：
10.1051/parasite/2018055
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Wen H
中科院分区：
文献类型：
--
作者：
Wang H;Li J;Zhang C;Guo B;Wei Q;Li L;Yang N;Peter McManus D;Gao X;Zhang W;Wen H
Cystic echinococcosis (CE) is a cosmopolitan parasitic disease caused by infection with the larval stage of Echinococcus granulosus sensu lato. Thioredoxin peroxidase (TPx) may play an essential role in the antioxidant defence system of E. granulosus s.l. as neither catalase nor glutathione peroxidase activities have been detected in the parasite. However, it is not known whether TPx affects the survival and growth of E. granulosus s.l. during development. In this study, three fragments of siRNA specific for EgTPx (siRNA-1/2/3) were designed and transfected into protoscoleces of E. granulosus sensu stricto by electroporation. Quantitative real-time PCR and Western blotting analysis showed that siRNA-3 significantly reduced the expression of EgTPx. Coincidentally, knockdown of EgTPx expression in protoscoleces with siRNA-3 significantly reduced the viability of the parasite under oxidative stress induced by 0.6 mM H2O2. In vitro culture studies showed that protoscoleces treated with siRNA-3 reduced pre-microcyst formation. In vivo experiments showed that injecting mice intraperitoneally with protoscoleces treated with siRNA-3 resulted in a significant reduction in the number, size and weight of CE cysts compared with those of control animals. Silencing of EgTPx led to the impairment of growth of E. granulosus s.s. both in vitro and in vivo, indicating that EgTPx is an important factor for protoscoleces survival and plays an important role in the antioxidant defence against the host during development.
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影响因子：
1.5
作者：
Boyle, JP;Wu, XJ;Yoshino, TP
通讯作者：
Yoshino, TP
影响因子：
4.8
作者：
Bonilla, Mariana;Denicola, Ana;Salinas, Gustavo
通讯作者：
Salinas, Gustavo
随着囊性棘球菌病的进展，甲状腺棘球菌的免疫蛋白质组谱的变化。
影响因子：
3.2
作者：
Ahn CS;Han X;Bae YA;Ma X;Kim JT;Cai H;Yang HJ;Kang I;Wang H;Kong Y
通讯作者：
Kong Y
影响因子：
11.8
作者：
Gavidia CM;Gonzalez AE;Zhang W;McManus DP;Lopera L;Ninaquispe B;Garcia HH;Rodríguez S;Verastegui M;Calderon C;Pan WK;Gilman RH
通讯作者：
Gilman RH
影响因子：
3.3
作者：
Monteiro, Karina M.;Lorenzatto, Karina R.;Ferreira, Henrique B.
通讯作者：
Ferreira, Henrique B.