Timing of peripheral blood stem cell yield: comparison of alternative methods with the classic method for CD34+ cell determination.
Timing of peripheral blood stem cell yield: comparison of alternative methods with the classic method for CD34+ cell determination.
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DOI：
10.1155/2014/575368
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发表时间：
2014
影响因子：
--
通讯作者：
Zak P
中科院分区：
文献类型：
--
作者：
Fatorova I;Blaha M;Lanska M;Vokurkova D;Rezacova V;Zak P
造血干细胞（HSCs），仍然代表着生物学上的某种奥秘，具有分裂成相等细胞并重新填充造血组织的独特性质。这种潜力使其能够用于移植治疗。通过流式细胞术测定CD 34+细胞来评估用于移植的HSC移植物的质量，这使得第一次单采的最佳时机和外周血干细胞（PBSC）的最大产量的获得成为可能。为了确定一种更有效的评估CD 34+细胞的方法，我们将以下替代方法与参考方法进行了比较：造血祖细胞（HPC）计数（使用Sysmex XE-2100分析仪）、CD 133+细胞检测和PBSC中醛脱氢酶活性定量。评价了266例单采术（84例患者）。在前体血液中，新方法产生的数据与参考方法一致。ROC曲线显示，对于第一天单采目标，HPC方法的最佳预测截止值为0.032个细胞/mL（灵敏度73.4%，特异性69.3%）。与其他测试方法相比，HPC方法表现出一定的实用优势。HPC计数法可作为确定首次单采时机的辅助方法，具有简便、快速、廉价等优点。
Hematopoietic stem cells (HSCs), still represent a certain mystery in biology, have a unique property of dividing into equal cells and repopulating the hematopoietic tissue. This potential enables their use in transplantation treatments. The quality of the HSC grafts for transplantation is evaluated by flow cytometric determination of the CD34+ cells, which enables optimal timing of the first apheresis and the acquisition of maximal yield of the peripheral blood stem cells (PBSCs). To identify a more efficient method for evaluating CD34+ cells, we compared the following alternative methods with the reference method: hematopoietic progenitor cells (HPC) enumeration (using the Sysmex XE-2100 analyser), detection of CD133+ cells, and quantification of aldehyde dehydrogenase activity in the PBSCs. 266 aphereses (84 patients) were evaluated. In the preapheretic blood, the new methods produced data that were in agreement with the reference method. The ROC curves have shown that for the first-day apheresis target, the optimal predictive cut-off value was 0.032 cells/mL for the HPC method (sensitivity 73.4%, specificity 69.3%). HPC method exhibited a definite practical superiority as compared to other methods tested. HPC enumeration could serve as a supplementary method for the optimal timing of the first apheresis; it is simple, rapid, and cheap.
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影响因子：
4.5
作者：
Lee JL;Kim SB;Lee GW;Ryu MH;Kim EK;Kim S;Kim WK;Lee JS;Park KU;Suh C
通讯作者：
Suh C
影响因子：
6.5
作者：
Pollard, Y;Watts, MJ;Machin, SJ
通讯作者：
Machin, SJ
DOI：
10.1073/pnas.96.16.9118
发表时间：
1999-08-03
影响因子：
11.1
作者：
Storms, RW;Trujillo, AP;Smith, C
通讯作者：
Smith, C
影响因子：
20.3
作者：
Storms, RW;Green, PD;Smith, CA
通讯作者：
Smith, CA
影响因子：
4.8
作者：
Yu, J;Leisenring, M;Rowley, SD
通讯作者：
Rowley, SD