Clinical usefulness of the hematopoietic progenitor cell counts in predicting the optimal timing of peripheral blood stem cell harvest.
Clinical usefulness of the hematopoietic progenitor cell counts in predicting the optimal timing of peripheral blood stem cell harvest.
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DOI：
10.3346/jkms.2003.18.1.27
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发表时间：
2003-02
影响因子：
4.5
通讯作者：
Suh C
中科院分区：
文献类型：
--
作者：
Lee JL;Kim SB;Lee GW;Ryu MH;Kim EK;Kim S;Kim WK;Lee JS;Park KU;Suh C
虽然外周血(PB)中CD34+细胞的计数可以预测收集到的细胞数量，但所使用的流式细胞仪技术复杂且昂贵，需要几个小时才能在临床实践环境中获得结果。Sysmex SE-9000自动血细胞分析仪提供未成熟细胞的估计，称为造血祖细胞(HPC)。本研究的目的是评估HPC在预测外周血祖细胞(PBPC)采集最佳时间方面的临床应用价值。本文对160例血液病或实体恶性肿瘤患者的628例遗体进行了研究。用Spearman等级统计法评估HPC、WBC、单个核细胞(MNC)和CD34+细胞之间的相关性。绘制受者工作特征(ROC)曲线，计算不同目标CD34+细胞集落数的HPC分界值预测值。与外周血白细胞和外周血单核细胞相比，外周血HPC与采集的CD34+细胞的相关性更强(Rho=0.592，p<；0.001)。ROC曲线显示，以50×10(6)/L为靶细胞或1×10(6)/kg，敏感度为75%。HPC和GT；或=50×10(6)/L对CD34+细胞和GT；或=1×10(6)/kg的阳性预测值为59.7%，阴性预测值为81.1%。在临床实践中，应用可变的HPC截断值将是预测PBPC采集的最佳时机的有用工具。
Although enumeration of CD34+ cells in the peripheral blood (PB) on the day of apheresis predicts the quantity of those cells collected, the flow cytometric techniques used are complex and expensive, and several hours are required to obtain the result in the clinical practice setting. The Sysmex SE-9000 automated haematology analyzer provides an estimate of immature cells, called hematopoietic progenitor cells (HPC). The aim of this study was to evaluate the clinical usefulness of HPC in predicting the optimal timing of peripheral blood progenitor cells (PBPC) harvest. Studies were performed on 628 aphereses from 160 patients with hematologic or solid malignancies. Spearman's rank statistics was used to assess correlation between HPC, WBC, mononuclear cells (MNC), and CD34+ cells. A receiver operating characteristic (ROC) curve was drawn for cutoff value of HPC, and predictive values of the chosen cutoff value of HPC for different target CD34+ cell collections were calculated. The PB HPC had a stronger correlation (rho=0.592, p<0.001) with collected CD34+ cells than did PB WBC and PB MNC. The ROC curve showed that the best cutoff value of HPC was 50 x 10(6)/L for the target CD34+ cells > or =1 x 10(6)/kg with sensitivity of 75%. Positive and negative predictive values of HPC > or =50 x 10(6)/L for CD34+ cells > or =1 x 10(6)/kg were 59.7% and 81.1%, respectively. In the clinical practice setting, applying variable cutoff values of HPC would be a useful tool to predict the optimal timing of PBPC collection.
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影响因子：
20.3
作者：
WEAVER, CH;HAZELTON, B;WEST, W
通讯作者：
WEST, W
影响因子：
20.3
作者：
DREGER, P;KLOSS, M;SCHMITZ, N
通讯作者：
SCHMITZ, N
影响因子：
6.5
作者：
Pollard, Y;Watts, MJ;Machin, SJ
通讯作者：
Machin, SJ
影响因子：
4.8
作者：
Armitage, S;Hargreaves, R;Navarrete, C
通讯作者：
Navarrete, C
DOI：
10.1089/scd.1.1997.6.575
发表时间：
1997-12-01
期刊：
JOURNAL OF HEMATOTHERAPY
影响因子：
--
作者：
Luider, J;Brown, C;Russell, JA
通讯作者：
Russell, JA