Paper spray ionization devices for direct, biomedical analysis using mass spectrometry.
Paper spray ionization devices for direct, biomedical analysis using mass spectrometry.
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DOI：
10.1016/j.ijms.2011.05.013
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发表时间：
2012-02-15
影响因子：
1.8
通讯作者：
Ouyang Z
中科院分区：
文献类型：
--
作者：
Yang Q;Wang H;Maas JD;Chappell WJ;Manicke NE;Cooks RG;Ouyang Z
纸喷雾离子化是一种直接、快速、低成本的采样和离子化方法，可用于复杂混合物的定性和定量质谱分析。通过在多孔基质上施加高电压和少量（约10 μL）喷雾溶剂生成分析物离子。样品可以预先加载到纸上或混合到喷雾溶液中。纸的几何形状和提供必要内标物的方法是影响电离效率以及随后分析数据的灵敏度和定量准确度的重要因素。当纸尖端的切割角度改变时，喷雾羽流、总喷雾电流和尖端处的电场强度都相应地改变，从而导致信号强度的差异。进样量是获得稳定的MS信号和准确定量结果的另一个重要因素。最佳的样品负载被发现是依赖于纸张的大小。溶解和喷雾过程也进行了研究，并在纸上的分析物转移被证明是在很大程度上与散装溶液流向喷嘴。从这些系统性研究中收集的信息为纸喷雾MS的一次性样品盒的设计和优化提供了指导，该装置可能适用于快速临床分析，特别是床旁诊断。
Paper spray ionization has been developed as a direct, fast and low-cost sampling and ionization method for qualitative and quantitative mass spectrometric (MS) analysis of complex mixtures. Analyte ions are generated by applying a high voltage and a small volume (~10 μL) of spray solvent onto a porous substrate. The sample can be preloaded onto the paper or mixed into the spray solution. The geometry of the paper and the method of supplying the necessary internal standard are important factors that affect the ionization efficiency and subsequently the sensitivity and quantitation accuracy of the analytical data. As the cut angle of the paper tip is changed, the spray plume, the total spray current and the electric field intensity at the tip all vary correspondingly, with resulting differences in signal intensity. Sample load is another important factor for obtaining a stable MS signal and accurate quantitative results. The optimal sample load was found to be dependent on the paper size. The dissolution and spray process was also investigated and analyte transfer on paper was shown to be largely associated with bulk solution flow towards the spray tip. The information gathered from these systematic studies provides guidance for the design and optimization of a disposable sample cartridge for paper spray MS, a device which potentially is suitable for fast clinical analysis, especially for point-of-care diagnostics.
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