Chromatin-associated SUMOylation controls the transcriptional switch between plant development and heat stress responses.
Chromatin-associated SUMOylation controls the transcriptional switch between plant development and heat stress responses.
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染色质相关 SUMO 化控制植物发育和热应激反应之间的转录转换
DOI：
10.1016/j.xplc.2020.100091
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发表时间：
2021-01-11
影响因子：
10.5
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Han D;Chen C;Xia S;Liu J;Shu J;Nguyen V;Lai J;Cui Y;Yang C
翻译后蛋白质修饰被称为SUMO化在各种物种的热应激反应中具有保守的作用。植物细胞中基因表达的全局调控和染色质相关的SUMO化之间的功能联系尚不清楚。在这里，我们发现了在室温下生长的拟南芥中，染色质相关的SUMO化和转录开关之间的全基因组关系，暴露于热应激，暴露于热应激后恢复。以全基因组ChIP-seq为特征的小泛素样修饰物（SUMO）相关染色质位点通常与活性染色质标记物相关。在热应激反应中，与染色质相关的SUMO信号在启动子转录起始位点区域增加，而在基因体中减少。RNA-seq分析支持了染色质相关SUMO化在高温快速反应过程中的转录激活作用。染色质上SUMO信号的变化与热响应基因的上调和生长相关基因的下调有关。SUMO连接酶基因SIZ 1的破坏废除了染色质上的SUMO信号，并减弱了对热应激的快速转录反应。SUMO信号峰富集在不同温度条件下由不同组的转录因子识别的DNA元件中。这些观察结果提供了证据，染色质相关的SUMO化调节植物细胞发育和热胁迫反应之间的转录开关。拟南芥全基因组ChIP-seq揭示了SUMO相关信号在染色质上的分布响应于热胁迫而改变，并且与基因转录的激活和抑制相关。这种类型的染色质相关的SUMO化主要依赖于SUMO连接酶SIZ 1，并控制植物发育和热胁迫反应之间的转录开关。
The post-translational protein modification known as SUMOylation has conserved roles in the heat stress responses of various species. The functional connection between the global regulation of gene expression and chromatin-associated SUMOylation in plant cells is unknown. Here, we uncovered a genome-wide relationship between chromatin-associated SUMOylation and transcriptional switches in Arabidopsis thaliana grown at room temperature, exposed to heat stress, and exposed to heat stress followed by recovery. The small ubiquitin-like modifier (SUMO)-associated chromatin sites, characterized by whole-genome ChIP-seq, were generally associated with active chromatin markers. In response to heat stress, chromatin-associated SUMO signals increased at promoter-transcriptional start site regions and decreased in gene bodies. RNA-seq analysis supported the role of chromatin-associated SUMOylation in transcriptional activation during rapid responses to high temperature. Changes in SUMO signals on chromatin were associated with the upregulation of heat-responsive genes and the downregulation of growth-related genes. Disruption of the SUMO ligase gene SIZ1 abolished SUMO signals on chromatin and attenuated rapid transcriptional responses to heat stress. The SUMO signal peaks were enriched in DNA elements recognized by distinct groups of transcription factors under different temperature conditions. These observations provide evidence that chromatin-associated SUMOylation regulates the transcriptional switch between development and heat stress response in plant cells. Whole-genome ChIP-seq in Arabidopsis reveals that the distribution of SUMO-associated signals on chromatin changes in response to heat stress and is associated with the activation and repression of gene transcription. This type of chromatin- associated SUMOylation depends primarily on SUMO ligase SIZ1 and controls the transcriptional switch between plant development and heat stress responses.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
18
作者：
Ezer D;Jung JH;Lan H;Biswas S;Gregoire L;Box MS;Charoensawan V;Cortijo S;Lai X;Stöckle D;Zubieta C;Jaeger KE;Wigge PA
通讯作者：
Wigge PA
影响因子：
16
作者：
Heinz S;Benner C;Spann N;Bertolino E;Lin YC;Laslo P;Cheng JX;Murre C;Singh H;Glass CK
通讯作者：
Glass CK
影响因子：
11.6
作者：
Catala, Rafael;Ouyang, Jian;Chua, Nam-Hai
通讯作者：
Chua, Nam-Hai
拟南芥 ARGONAUTE 1 结合染色质促进基因转录以响应激素和应激
影响因子：
11.8
作者：
Liu, Chang;Xin, Ying;Qi, Yijun
通讯作者：
Qi, Yijun