Quantitation of cell-associated carbon nanotubes: selective binding and accumulation of carboxylated carbon nanotubes by macrophages.
Quantitation of cell-associated carbon nanotubes: selective binding and accumulation of carboxylated carbon nanotubes by macrophages.
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DOI：
10.1080/17435390.2018.1472309
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发表时间：
2018-09
期刊：
影响因子：
5
通讯作者：
Draper R
中科院分区：
文献类型：
--
作者：
Wang R;Lee M;Kinghorn K;Hughes T;Chuckaree I;Lohray R;Chow E;Pantano P;Draper R
为了理解羧化对碳纳米管与细胞的相互作用的影响，通过定量从细胞提取的CNT来测量由巨噬细胞积累的用Pluronic® F-108涂覆的原始多壁碳纳米管（P-MWNT）或羧化多壁碳纳米管（C-MWNT）的量。小鼠RAW 264.7巨噬细胞和分化的人THP-1（dTHP-1）巨噬细胞在37 °C下暴露24小时期间积累的C-MWNT是P-MWNT的80-100倍。RAW 264.7细胞的C-MWNTs的积累是不致命的，但是，吞噬作用受损，因为随后的聚苯乙烯珠的摄取减少后，20小时暴露于C-MWNTs。C-MWNTs的选择性聚集表明巨噬细胞上可能存在与C-MWNTs结合的受体。C-MWNT与巨噬细胞的结合在4 °C下在不存在血清的情况下测量为浓度的函数，以最小化血清蛋白或温度依赖性摄取过程的潜在干扰。结果是，细胞结合的C-MWNTs是P-MWNTs的8.7倍，这与C-MWNTs在37 °C下的选择性积累一致。此外，血清强烈拮抗C-MWTS与巨噬细胞的结合，表明血清中含有结合抑制剂。此外，A类清道夫受体（SR-As）的抑制剂减少了约50%的C-MWNTs的结合，表明SR-As有助于C-MWNTs在巨噬细胞中的结合和内吞，但其他受体也可能参与。总之，证据支持巨噬细胞含有对C-MWNT具有选择性的结合位点的假设，与P-MWNT相比，其促进C-MWNT的高积累。
To understand the influence of carboxylation on the interaction of carbon nanotubes with cells, the amount of pristine multi-walled carbon nanotubes (P-MWNTs) or carboxylated multi-walled carbon nanotubes (C-MWNTs) coated with Pluronic® F-108 that were accumulated by macrophages was measured by quantifying CNTs extracted from cells. Mouse RAW 264.7 macrophages and differentiated human THP-1 (dTHP-1) macrophages accumulated 80–100 times more C-MWNTs than P-MWNTs during a 24-hour exposure at 37 °C. The accumulation of C-MWNTs by RAW 264.7 cells was not lethal; however, phagocytosis was impaired as subsequent uptake of polystyrene beads was reduced after a 20-h exposure to C-MWNTs. The selective accumulation of C-MWNTs suggested that there might be receptors on macrophages that bind C-MWNTs. The binding of C-MWNTs to macrophages was measured as a function of concentration at 4 °C in the absence of serum to minimize the potential interference by serum proteins or temperature-dependent uptake processes. The result was that the cells bound 8.7 times more C-MWNTs than P-MWNTs, consistent with the selective accumulation of C-MWNTs at 37 °C. In addition, serum strongly antagonized the binding of C-MWTS to macrophages, suggesting that serum contained inhibitors of binding. Moreover, inhibitors of class A scavenger receptor (SR-As) reduced the binding of C-MWNTs by about 50%, suggesting that SR-As contribute to the binding and endocytosis of C-MWNTs in macrophages but that other receptors may also be involved. Altogether, the evidence supports the hypothesis that macrophages contain binding sites selective for C-MWNTs that facilitate the high accumulation of C-MWNTs compared to P-MWNTs.
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