B cell subsets in adult-onset Still's disease: potential candidates for disease pathogenesis and immunophenotyping.
B cell subsets in adult-onset Still's disease: potential candidates for disease pathogenesis and immunophenotyping.
复制标题
DOI：
10.1186/s13075-023-03070-2
复制
发表时间：
2023-06-15
影响因子：
4.9
通讯作者：
中科院分区：
文献类型：
--
作者：
成人发病斯蒂尔氏病（AOSD）是一种病因不明的全身性自身炎症性疾病。B细胞是不同风湿病的关键参与者，它们在AOSD中的作用很少被研究。本研究旨在揭示AOSD的B细胞亚群特征，为AOSD的B细胞诊断和靶向治疗提供依据。流式细胞术检测AOSD患者和健康对照（hc）外周血B细胞亚群。首先，比较了B细胞亚群的频率。然后进行相关性分析，探讨B细胞亚群与AOSD临床表现的相关性。最后，采用无偏分层聚类法将AOSD患者按照不同的B细胞亚群特征分为三组，比较三组患者的临床特征。AOSD患者B细胞亚群频率发生改变。AOSD患者外周血中促进疾病的亚群（如naïve B细胞、双阴性B细胞（DN B细胞）和浆母细胞）增加，而潜在的调节亚群（如未开关记忆B细胞（UM B细胞）和CD24hiCD27+ B细胞（B10细胞））减少。此外，AOSD中B细胞亚群的改变与临床和免疫学特征相关，如免疫细胞、凝血特征和肝酶。有趣的是，AOSD患者可分为具有不同B细胞免疫表型的三组：1组（naïve B细胞为主），2组（CD27+记忆B细胞为主）和3组（自身抗体产生浆细胞的前体为主）。此外，这三组患者表现出不同的表现，包括免疫细胞、肝或心肌酶、凝血特征和全身评分。AOSD患者的B细胞亚群显著改变，可能与疾病发病机制有关。这些发现将启发基于B细胞的诊断和针对这种难治性疾病的靶向治疗。在线版本包含补充材料，可在10.1186/s13075-023-03070-2获得。
Adult-onset Still’s disease (AOSD) is a systemic autoinflammatory disorder of unknown etiology. B cells are critical participants in different rheumatic diseases, and their roles in AOSD are rarely investigated. This study aimed to unveil the B cell subset features in AOSD and provide evidence for B cell-based diagnosis and targeted therapies of AOSD. B cell subsets in the peripheral blood of AOSD patients and healthy controls (HCs) were detected by flow cytometry. Firstly, the frequencies of B cell subsets were compared. Then, the correlation analysis was performed to explore the correlation between B cell subsets and clinical manifestations in AOSD. Finally, unbiased hierarchical clustering was performed to divide AOSD patients into three groups with different B cell subset features, and the clinical characteristics of the three groups were compared. The frequencies of B cell subsets were altered in AOSD patients. Disease-promoting subsets (such as naïve B cells, double negative B cells (DN B cells), and plasmablasts) increased, and potential regulatory subsets (such as unswitched memory B cells (UM B cells) and CD24hiCD27+ B cells (B10 cells)) decreased in the peripheral blood of AOSD patients. In addition, the altered B cell subsets in AOSD correlated with the clinical and immunological features, such as immune cells, coagulation features, and liver enzymes. Intriguingly, AOSD patients could be divided into three groups with distinct B cell immunophenotyping: group 1 (naïve B cells-dominant), group 2 (CD27+ memory B cells-dominant), and group 3 (precursors of autoantibody-producing plasma cells-dominant). Moreover, these three group patients demonstrated differential manifestations, including immune cells, liver or myocardial enzymes, coagulation features, and systemic score. B cell subsets are significantly altered in AOSD patients, potentially contributing to the disease pathogenesis. These findings would inspire B cell-based diagnosis and targeted therapies for this refractory disease. The online version contains supplementary material available at 10.1186/s13075-023-03070-2.
登录
查看更多内容
类风湿关节炎中基因特征改变的 CD27( )IgD( ) B 细胞受损
影响因子：
7.3
作者：
Hu F;Zhang W;Shi L;Liu X;Jia Y;Xu L;Zhu H;Li Y;Xu D;Lu L;Qiu X;Liu W;Qiao J;Wang Y;Li Z
通讯作者：
Li Z
影响因子：
5.1
作者：
Marston B;Palanichamy A;Anolik JH
通讯作者：
Anolik JH
DOI：
10.1016/j.clinre.2021.101698
发表时间：
2021-05-01
影响因子：
2.7
作者：
Cardoso, Chandra Chiappin;Matiollo, Camila;Santos-Silva, Maria Claudia
通讯作者：
Santos-Silva, Maria Claudia
影响因子：
3.9
作者：
Rau, Monika;Schiller, Martin;Blank, Norbert
通讯作者：
Blank, Norbert
DOI：
10.1084/jem.188.9.1679
发表时间：
1998-11-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Klein U;Rajewsky K;Küppers R
通讯作者：
Küppers R