Plasmodium vivax population structure and transmission dynamics in Sabah Malaysia.
Plasmodium vivax population structure and transmission dynamics in Sabah Malaysia.
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DOI：
10.1371/journal.pone.0082553
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Auburn S
中科院分区：
文献类型：
--
作者：
Abdullah NR;Barber BE;William T;Norahmad NA;Satsu UR;Muniandy PK;Ismail Z;Grigg MJ;Jelip J;Piera K;von Seidlein L;Yeo TW;Anstey NM;Price RN;Auburn S
尽管马来西亚在控制疟疾方面取得了重大进展，但间日疟原虫复杂的传播动态继续对国家消灭疟疾的努力构成挑战。为了评估持续干预对沙巴间日疟原虫传播动力学的影响，我们对来自沙巴的97株（8株复发）中的9个短串联重复序列标记进行了基因分型，重点是两个地区，科塔Marudu（KM，n = 24）和亚庇（KK，n = 21），为期2年。 对沙巴州范围内数据集的结构分析显示了多个亚群。KM和KK之间观察到显著差异（F ST =0.243），仅相距130 Km。 与低地方性传播相一致，感染的复杂性在KM（平均MOI = 1.38）和KK（平均MOI = 1.19）都是适度的。 然而，种群多样性保持中等（HE = 0.583 KM和HE = 0.667 KK）。 时间趋势表明，反映流行病传播动态的克隆扩张。这些分离株的单倍型频率随着时间的推移而下降，但在整个研究期间持续低频率。在KM和KK中检测到一系列不同的低频分离株，其中一些可能反映了以前扩张的残留物。与克隆扩增一致，当相同的单倍型出现一次时，高水平的连锁不平衡（KK和KM中的I A S >0.5 [P<0.0001]）急剧下降（KM中的I A S = 0.07 [P = 0.0076]，KK中的I A S =-0.003 [P = 0.606]）。 所有8例复发（可能是复发）均与既往感染同源。这些复发可能会促进寄生虫谱系的持久性，维持当地的多样性。总之，沙巴间日疟原虫种群的减少似乎使这种低流行性的环境容易受到流行扩张的影响。移徙可能在引入新的寄生虫株导致流行病扩大方面发挥重要作用，对消灭疟疾产生重要影响。
Despite significant progress in the control of malaria in Malaysia, the complex transmission dynamics of P. vivax continue to challenge national efforts to achieve elimination. To assess the impact of ongoing interventions on P. vivax transmission dynamics in Sabah, we genotyped 9 short tandem repeat markers in a total of 97 isolates (8 recurrences) from across Sabah, with a focus on two districts, Kota Marudu (KM, n = 24) and Kota Kinabalu (KK, n = 21), over a 2 year period. STRUCTURE analysis on the Sabah-wide dataset demonstrated multiple sub-populations. Significant differentiation (F ST = 0.243) was observed between KM and KK, located just 130 Km apart. Consistent with low endemic transmission, infection complexity was modest in both KM (mean MOI = 1.38) and KK (mean MOI = 1.19). However, population diversity remained moderate (H E = 0.583 in KM and H E = 0.667 in KK). Temporal trends revealed clonal expansions reflecting epidemic transmission dynamics. The haplotypes of these isolates declined in frequency over time, but persisted at low frequency throughout the study duration. A diverse array of low frequency isolates were detected in both KM and KK, some likely reflecting remnants of previous expansions. In accordance with clonal expansions, high levels of Linkage Disequilibrium (I A S >0.5 [P<0.0001] in KK and KM) declined sharply when identical haplotypes were represented once (I A S = 0.07 [P = 0.0076] in KM, and I A S = -0.003 [P = 0.606] in KK). All 8 recurrences, likely to be relapses, were homologous to the prior infection. These recurrences may promote the persistence of parasite lineages, sustaining local diversity. In summary, Sabah's shrinking P. vivax population appears to have rendered this low endemic setting vulnerable to epidemic expansions. Migration may play an important role in the introduction of new parasite strains leading to epidemic expansions, with important implications for malaria elimination.
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3.8
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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Yeo, Tsin W.
DOI：
10.1111/j.1471-8286.2006.01534.x
发表时间：
2007-01-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Karunaweera, N. D.;Ferreira, M. . U.;Wirth, D. F.
通讯作者：
Wirth, D. F.