Cloning and sequencing of the matrix protein (M) gene of turkey rhinotracheitis virus reveal a gene order different from that of respiratory syncytial virus.
Cloning and sequencing of the matrix protein (M) gene of turkey rhinotracheitis virus reveal a gene order different from that of respiratory syncytial virus.
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DOI：
10.1016/0042-6822(92)90007-c
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发表时间：
1992-02
期刊：
影响因子：
3.7
通讯作者：
Cavanagh D
中科院分区：
文献类型：
--
作者：
Yu Q;Davis PJ;Li J;Cavanagh D
火鸡鼻气管炎病毒（TRTV）的一些生化特性和融合糖蛋白(F)的序列表明，TRTV是副粘病毒科肺炎病毒亚科的一种肺炎病毒。由于已知TRTV可以产生多顺反子mrna，因此利用F基因区域对应的寡核苷酸引物从TRTV菌株U K/3 BV/85感染的Vero细胞mrna中生成cDNA。4个cdna的测序结果显示，F基因开始（3′端）附近的基因为基质(M)蛋白的基因，即TRTV的部分基因序为3′-M-F-5′。这是出乎意料的，因为人类呼吸道合胞病毒（RS）是肺炎病毒属的型种，其部分基因序列为3 ' -M-SH-G-F-5 '，其中SH和G分别是小疏水蛋白和附着糖蛋白。相反，TRTV与副粘病毒科（副粘病毒亚科）的麻疹病毒属和副粘病毒属相似，它们的部分基因顺序为3 ‘ -M-F-5 ’。另外两个寡核苷酸，一个对应于M基因末端附近的序列，另一个（寡核苷酸B）对应于F基因开头附近的序列，它们的5 '端在cDNA序列的基础上相隔300个核苷酸，以基因组RNA为模板用于聚合酶链反应（PCR）。只得到0.3 kb的PCR产物。从鸡气管器官培养的另外3株TRTV菌株（SA/91 /78、UK/8544/85和SA/2381/88）中，利用这些寡核苷酸和基因组RNA也获得了相同大小的产物。此外，利用TRTV-3BV和SA/2381/88的基因组RNA进行PCR，其中寡核苷酸B和另一个寡核苷酸位于基因M上游的5 '端附近，根据序列数据间隔1141个核苷酸。只得到1.14 kb的PCR产物。如果在M和F之间有另一个基因，那么预计会有更大的产物。没有这样大的产物，再加上感染细胞含有M-F双双子mrna的证明，支持了TRTV基因组中M基因与F基因相邻的3‘-M-F-5 ’数量级的结论。M和F mRNA的5 '端通过mRNA作图确认。TRTV M基因编码的蛋白含有254个氨基酸，与RS病毒的蛋白非常相似（256个残基；37%的氨基酸同源性），但与麻疹病毒和副粘病毒的蛋白非常不同（约350个残基）。因此，根据M和F基因的序列，TRTV与肺炎病毒的关系比与副粘病毒和麻疹病毒的关系更密切。
Several biochemical properties and the sequence of the fusion glycoprotein (F) have indicated that turkey rhinotracheitis virus (TRTV) is a pneumovirus, subfamily Pneumovirinae of the Paramyxoviridae family. As TRTV was known to generate polycistronic mRNAs, cDNA was generated from TRTV strain U K/3 BV/85-infected Vero cell mRNAs using an oligonucleotide primer corresponding to a region of the F gene. Sequencing of four cDNAs revealed that the gene adjacent to the beginning (3′ end) of the F gene was that for the matrix (M) protein, i.e., that TRTV had the partial gene order 3′-M-F-5′. This was unexpected as human respiratory syncytial (RS) virus, the type species of the genus Pneumovirus, has the partial gene order 3′-M-SH-G-F-5′, where SH and G are the small hydrophobic protein and attachment glycoprotein, respectively. Instead TRTV resembled the Morbillivirus and Paramyxovirus genera of the Paramyxoviridae (subfamily Paramyxoviridae) which have the partial gene order 3′-M-F-5′. Two further oligonucleotides, one corresponding to a sequence near the end of the M gene and the other (oligo B) to a sequence near the beginning of the F gene, with their 5′ ends spaced 300 nucleotides apart on the basis of the cDNA sequence, were used in a polymerase chain reaction (PCR) using genomic RNA as template. Only a PCR product of 0.3 kb was obtained. The same sized product was also obtained using these oligonucleotides and genomic RNA from three other TRTV strains (SA/91 /78, UK/8544/85, and SA/2381/88) which had been grown in chicken tracheal organ cultures. In addition PCR was performed using genomic RNA from TRTV-3BV and SA/2381/88 with oligo B and another oligonucleotide near the 5′ end of the gene upstream from M, spaced 1141 nucleotides apart on the basis of the sequence data. Only a 1.14-kb PCR product was obtained. Larger products would have been expected if another gene had been situated between M and F. The absence of such larger products, plus the demonstration that infected cells contained M-F dicistronic mRNAs, supported the conclusion that in the TRTV genome the M gene is adjacent to the F gene in the order 3'-M-F-5′. The 5′ termini of the M and F mRNAs were confirmed by mRNA mapping. The TRTV M gene encoded a protein of 254 amino acids, very similar to that of RS virus (256 residues; 37% amino acid identity) but very different from that of the morbilliviruses and paramyxoviruses (approximately 350 residues). Thus, on the basis of the sequences of both the M and the F genes, TRTV is more closely related to the pneumoviruses than to the paramyxoviruses and morbilliviruses.
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影响因子：
2.7
作者：
COOK, JKA;DARBYSHIRE, JH;PETERS, RW
通讯作者：
PETERS, RW
影响因子：
3.7
作者：
BUECHI, M;BACHI, T
通讯作者：
BACHI, T
影响因子：
5
作者：
ELLIOTT, GD;AFZAL, MA;RIMA, BK
通讯作者：
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影响因子：
5.4
作者：
GIUFFRE, RM;TOVELL, DR;TYRRELL, DLJ
通讯作者：
TYRRELL, DLJ
影响因子：
5.4
作者：
BELLINI, WJ;ENGLUND, G;LAZZARINI, RA
通讯作者：
LAZZARINI, RA