Arginine Methyltransferases as Regulators of RNA-Binding Protein Activities in Pathogenic Kinetoplastids.
Arginine Methyltransferases as Regulators of RNA-Binding Protein Activities in Pathogenic Kinetoplastids.
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DOI：
10.3389/fmolb.2021.692668
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发表时间：
2021
影响因子：
5
通讯作者：
Walrad PB
中科院分区：
文献类型：
--
作者：
Campagnaro GD;Nay E;Plevin MJ;Cruz AK;Walrad PB
大量真核蛋白是通过单一或组合的翻译后共价修饰处理的，这可能改变它们的活性、相互作用和命运。每种蛋白质的一系列修饰可以被认为是一个“调控代码”。在PTMs中，由蛋白质精氨酸甲基转移酶（PRMTs）催化的精氨酸甲基化可以影响蛋白质与其他大分子（如核酸或其他蛋白质）的相互作用。事实上，许多rna结合蛋白（rbp）是PRMTs的靶标。rbp的甲基化状态可能影响其结合rna的表达，并影响多种生理和病理细胞过程。与大多数真核生物不同，着丝质体具有压倒性的无内含子基因，这些基因排列在多顺反子单元内，成熟的mrna通过反式剪接产生。因此，这些生物中的基因表达高度依赖于转录后控制，因此依赖于rbp的作用。这些遗传特征使锥虫成为研究基因表达转录后调控的优秀模型。在致病的着丝质体中，PRMTs在控制rbp活性中的作用已经被研究了近20年，近年来取得了重要进展。其中包括发现大约10%的布氏锥虫蛋白质组携带精氨酸甲基化，精氨酸甲基化控制利什曼原虫与宿主的相互作用。在此，我们回顾了锥虫PRMTs如何调节rbp的活性，包括通过调节与RNA和/或蛋白质复合物形成的相互作用，并讨论了这如何影响细胞和生物过程。我们进一步强调了锥虫prmt的独特结构特征及其如何有助于其单一功能。
A large number of eukaryotic proteins are processed by single or combinatorial post-translational covalent modifications that may alter their activity, interactions and fate. The set of modifications of each protein may be considered a “regulatory code”. Among the PTMs, arginine methylation, catalyzed by protein arginine methyltransferases (PRMTs), can affect how a protein interacts with other macromolecules such as nucleic acids or other proteins. In fact, many RNA-binding (RBPs) proteins are targets of PRMTs. The methylation status of RBPs may affect the expression of their bound RNAs and impact a diverse range of physiological and pathological cellular processes. Unlike most eukaryotes, Kinetoplastids have overwhelmingly intronless genes that are arranged within polycistronic units from which mature mRNAs are generated by trans-splicing. Gene expression in these organisms is thus highly dependent on post-transcriptional control, and therefore on the action of RBPs. These genetic features make trypanosomatids excellent models for the study of post-transcriptional regulation of gene expression. The roles of PRMTs in controlling the activity of RBPs in pathogenic kinetoplastids have now been studied for close to 2 decades with important advances achieved in recent years. These include the finding that about 10% of the Trypanosoma brucei proteome carries arginine methylation and that arginine methylation controls Leishmania:host interaction. Herein, we review how trypanosomatid PRMTs regulate the activity of RBPs, including by modulating interactions with RNA and/or protein complex formation, and discuss how this impacts cellular and biological processes. We further highlight unique structural features of trypanosomatid PRMTs and how it contributes to their singular functionality.
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影响因子：
3.3
作者：
Lott K;Li J;Fisk JC;Wang H;Aletta JM;Qu J;Read LK
通讯作者：
Read LK
影响因子：
4
作者：
Bedford, Mark T.
通讯作者：
Bedford, Mark T.
对真核生物的分类，命名法和多样性进行修订。
DOI：
10.1111/jeu.12691
发表时间：
2019-01
期刊：
The Journal of eukaryotic microbiology
影响因子：
--
作者：
Adl SM;Bass D;Lane CE;Lukeš J;Schoch CL;Smirnov A;Agatha S;Berney C;Brown MW;Burki F;Cárdenas P;Čepička I;Chistyakova L;Del Campo J;Dunthorn M;Edvardsen B;Eglit Y;Guillou L;Hampl V;Heiss AA;Hoppenrath M;James TY;Karnkowska A;Karpov S;Kim E;Kolisko M;Kudryavtsev A;Lahr DJG;Lara E;Le Gall L;Lynn DH;Mann DG;Massana R;Mitchell EAD;Morrow C;Park JS;Pawlowski JW;Powell MJ;Richter DJ;Rueckert S;Shadwick L;Shimano S;Spiegel FW;Torruella G;Youssef N;Zlatogursky V;Zhang Q
通讯作者：
Zhang Q
影响因子：
6.7
作者：
Erben ED;Fadda A;Lueong S;Hoheisel JD;Clayton C
通讯作者：
Clayton C
DOI：
10.1073/pnas.1525783113
发表时间：
2016-02-23
影响因子：
11.1
作者：
Debler, Erik W.;Jain, Kanishk;Stavropoulos, Pete
通讯作者：
Stavropoulos, Pete