Analysis of miRNAs Targeted Storage Regulatory Genes during Soybean Seed Development Based on Transcriptome Sequencing
Analysis of miRNAs Targeted Storage Regulatory Genes during Soybean Seed Development Based on Transcriptome Sequencing
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基于转录组测序的大豆种子发育过程中miRNA靶向贮藏调控基因分析
DOI：
10.3390/genes10060408
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发表时间：
2019-05
期刊：
影响因子：
3.5
通讯作者：
Zhaoming Qi
中科院分区：
文献类型：
--
作者：
Jingyao Yu;ZhanGuo Zhang;ShiYu Huang;Xue Han;Xinyu Wang;Wenjing Pan;Hongtao Qin;Huidong Qi;Zhengong Yin;Kexin Qu;Zexin Zhang;Shanshan Liu;Hongwei Jiang;Chunyan Liu;Zhenbang Hu;Xiaoxia Wu;Qingshan Chen;Daweixin;Zhaoming Qi
菜豆是一种重要的经济作物，被广泛用作植物蛋白和食用油的来源。微小RNA（microRNA，miRNA）是一类内源性小RNA，在进化保守的基因表达系统中起着重要的调控作用。本研究从穗农（SN 14）和ZYD 00006构建的染色体片段代换系（CSSL）中，选择4个表现型极端、蛋白质和油分含量高低的品系，种植并在籽粒发育的3个时期取样进行小RNA测序和表达分析。测序结果揭示了材料中miRNA的表达模式，预测了miRNA靶向调控基因，包括已知miRNA与靶基因的1967对对应关系，以及新miRNA与靶基因的597对对应关系。在筛选和注释了用于调控的靶基因后，鉴定了在种子发育期间差异表达的五个特定基因，随后分析了它们与miRNA的调控关系。通过实时定量PCR（RT-qPCR）验证靶基因的表达模式。我们的研究提供了更多关于大豆中miRNA调控网络的信息，并进一步确定了在大豆籽粒发育过程中调控贮藏的有用基因，为大豆品质性状的调控提供了理论依据。
Soybeans are an important cash crop and are widely used as a source of vegetable protein and edible oil. MicroRNAs (miRNA) are endogenous small RNA that play an important regulatory role in the evolutionarily conserved system of gene expression. In this study, we selected four lines with extreme phenotypes, as well as high or low protein and oil content, from the chromosome segment substitution line (CSSL) constructed from suinong (SN14) and ZYD00006, and planted and sampled at three stages of grain development for small RNA sequencing and expression analysis. The sequencing results revealed the expression pattern of miRNA in the materials, and predicted miRNA-targeted regulatory genes, including 1967 pairs of corresponding relationships between known-miRNA and their target genes, as well as 597 pairs of corresponding relationships between novel-miRNA and their target genes. After screening and annotating genes that were targeted for regulation, five specific genes were identified to be differentially expressed during seed development and subsequently analyzed for their regulatory relationship with miRNAs. The expression pattern of the targeted gene was verified by Real-time Quantitative PCR (RT-qPCR). Our research provides more information about the miRNA regulatory network in soybeans and further identifies useful genes that regulate storage during soy grain development, providing a theoretical basis for the regulation of soybean quality traits.
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影响因子：
12.3
作者：
Schrick K;Nguyen D;Karlowski WM;Mayer KF
通讯作者：
Mayer KF
影响因子：
4.4
作者：
Shamimuzzaman M;Vodkin L
通讯作者：
Vodkin L
影响因子：
--
作者：
Molly F. Thomas;K. Ansel
通讯作者：
Molly F. Thomas;K. Ansel
野生大豆 (Glycine soja Sieb.) 百粒重的 QTL 定位及上位效应分析
影响因子：
3.7
作者：
Xin D;Qi Z;Jiang H;Hu Z;Zhu R;Hu J;Han H;Hu G;Liu C;Chen Q
通讯作者：
Chen Q
影响因子：
14.9
作者：
Grant D;Nelson RT;Cannon SB;Shoemaker RC
通讯作者：
Shoemaker RC