PRC2 recruitment and H3K27me3 deposition at FLC require FCA binding of COOLAIR
PRC2 recruitment and H3K27me3 deposition at FLC require FCA binding of COOLAIR
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PRC2 招募和 H3K27me3 在 FLC 沉积需要 COOLAIR 的 FCA 结合
DOI：
10.1126/sciadv.aau7246
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发表时间：
2019-04
期刊：
影响因子：
13.6
通讯作者：
Yong Ding
中科院分区：
文献类型：
--
作者：
Yongke Tian;Han Zheng;Fei zhang;Shiliang Wang;Xiaoru Ji;Chao Xu;Yuehui He;Yong Ding
H3K27me3在FLC的沉积需要FCA-COOLAIR结合；Ssu72拮抗这种结合，从而减少H3K27me3在FLC的沉积。低温诱导的反义转录子COOLAIR通过增加H3K27me3和降低H3K36me3的转录水平来抑制开花基因C(FLC)的转录。然而，在没有冷处理的情况下，COOLAIR和组蛋白修饰因子之间的分子联系仍然不清楚。我们报道了RNA结合蛋白FCA与PRC2亚基卷叶(CLF)相互作用，并结合新生COOLAIR转录本，允许H3K27me3在FLC沉积。COOLAIR功能的丧失会导致FCA和CLF浓缩减少，进而降低FLC的H3K27me3水平。拟南芥蛋白磷酸酶Ssu72与FCA的RRM1基序物理上相互作用，从而拮抗FCA与COOLAIR的结合。Ssu72突变导致早花，FLC转录减少，CLF富集度和H3K27me3增加，FCA和COOLAIR亲和力增强。我们的结果表明，COOLAIR和Ssu72的FCA结合对于拟南芥中PRC2的浓缩和H3K27me3的沉积是至关重要的。
H3K27me3 deposition at FLC requires FCA-COOLAIR binding; SSU72 antagonizes this binding, thus reducing H3K27me3 deposition at FLC. The cold-induced antisense transcript COOLAIR represses FLOWERING LOCUS C (FLC) transcription with increased H3K27me3 and decreased H3K36me3 levels in response to cold temperatures. However, the molecular connection between COOLAIR and histone modification factors in the absence of cold treatment remains unclear. We report that the RNA binding protein FCA interacts with the PRC2 subunit CURLY LEAF (CLF) and binds nascent COOLAIR transcripts to allow deposition of H3K27me3 at FLC. Loss of COOLAIR function results in a reduction in FCA and CLF enrichment, which, in turn, decreases H3K27me3 levels at FLC. The Arabidopsis protein phosphatase SSU72 physically interacts with the RRM1 motif of FCA to antagonize FCA binding with COOLAIR. Mutations in SSU72 caused early flowering, reduced FLC transcription, increased CLF enrichment and H3K27me3, and enhanced affinity between FCA and COOLAIR. Our results suggest that FCA binding of COOLAIR and SSU72 is critical for PRC2 enrichment and H3K27me3 deposition in Arabidopsis.
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影响因子：
19
作者：
Böhmdorfer G;Wierzbicki AT
通讯作者：
Wierzbicki AT
影响因子：
16
作者：
Wei C;Xiao R;Chen L;Cui H;Zhou Y;Xue Y;Hu J;Zhou B;Tsutsui T;Qiu J;Li H;Tang L;Fu XD
通讯作者：
Fu XD
影响因子：
10.5
作者：
K. Xiang;J. Manley;L. Tong
通讯作者：
K. Xiang;J. Manley;L. Tong
CDKD;2 磷酸化 SPT5 是拟南芥中 VIP5 募集和正常开花所必需的。
影响因子：
11.6
作者：
Lu Chengyuan;Tian Yongke;Wang Shiliang;Su Yanhua;Mao Ting;Huang Tongtong;Chen Qingqing;Xu Zuntao;Ding Yong
通讯作者：
Ding Yong
DOI：
10.1073/pnas.0611459104
发表时间：
2007-02-27
影响因子：
11.1
作者：
Swiezewski, Szymon;Crevillen, Pedro;Dean, Caroline
通讯作者：
Dean, Caroline