Nanopore fingerprinting of supramolecular DNA nanostructures.
Nanopore fingerprinting of supramolecular DNA nanostructures.
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超分子DNA纳米结构的纳米孔指纹图谱。
DOI：
10.1016/j.bpj.2022.08.020
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发表时间：
2022-12-20
影响因子：
3.4
通讯作者：
Actis, Paolo
中科院分区：
文献类型：
--
作者：
Confederat, Samuel;Sandei, Ilaria;Mohanan, Gayathri;Walti, Christoph;Actis, Paolo
DNA纳米技术为新一代可编程纳米材料铺平了道路。利用DNA折纸技术，可以设计各种DNA结构，从单个瓦片到大规模、复杂、多瓦片阵列的自组装。这项技术依赖于数百个短DNA短链与一个长单链DNA支架的结合，该支架驱动定义良好的纳米结构的折叠。这样的DNA纳米结构在生物传感、药物传递和其他多功能材料中有了新的应用。在这项研究中，我们利用固态纳米孔的增强灵敏度，采用富含聚乙二醇的电解质，以单实体分辨率提供DNA折纸纳米结构高阶组件的实时、非破坏性和无标记指纹。该方法可以利用纳米结构诱导的等效电荷剩余作为判别因素，对复杂DNA折纸纳米结构的组装产率进行量化。我们用琼脂糖凝胶电泳和原子力显微镜成像比较了用纳米孔获得的四种超分子DNA纳米结构的组装率。我们证明了纳米孔系统可以在几分钟内提供复杂的超分子纳米结构的分析定量，而不需要任何标记和单分子分辨率。我们设想纳米孔检测平台可以应用于一系列纳米材料设计，并能够实时分析和操作大型DNA组装。
DNA nanotechnology has paved the way for new generations of programmable nanomaterials. Utilizing the DNA origami technique, various DNA constructs can be designed, ranging from single tiles to the self-assembly of large-scale, complex, multi-tile arrays. This technique relies on the binding of hundreds of short DNA staple strands to a long single-stranded DNA scaffold that drives the folding of well-defined nanostructures. Such DNA nanostructures have enabled new applications in biosensing, drug delivery, and other multifunctional materials. In this study, we take advantage of the enhanced sensitivity of a solid-state nanopore that employs a poly-ethylene glycol enriched electrolyte to deliver real-time, non-destructive, and label-free fingerprinting of higher-order assemblies of DNA origami nanostructures with single-entity resolution. This approach enables the quantification of the assembly yields for complex DNA origami nanostructures using the nanostructure-induced equivalent charge surplus as a discriminant. We compare the assembly yield of four supramolecular DNA nanostructures obtained with the nanopore with agarose gel electrophoresis and atomic force microscopy imaging. We demonstrate that the nanopore system can provide analytical quantification of the complex supramolecular nanostructures within minutes, without any need for labeling and with single-molecule resolution. We envision that the nanopore detection platform can be applied to a range of nanomaterial designs and enable the analysis and manipulation of large DNA assemblies in real time.
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影响因子：
38.3
作者：
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影响因子：
14.9
作者：
Lee AJ;Endo M;Hobbs JK;Davies AG;Wälti C
通讯作者：
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通讯作者：
Actis, Paolo
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通讯作者：
Keyser, Ulrich F.
影响因子：
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通讯作者：
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