Lifetime of glass nanopores in a PDMS chip for single-molecule sensing.
Lifetime of glass nanopores in a PDMS chip for single-molecule sensing.
复制标题
DOI：
10.1016/j.isci.2022.104191
复制
发表时间：
2022-05-20
期刊：
影响因子：
5.8
通讯作者：
Keyser, Ulrich F.
中科院分区：
文献类型：
--
作者：
Alawami, Mohammed F.;Boskovic, Filip;Zhu, Jinbo;Chen, Kaikai;Sandler, Sarah E.;Keyser, Ulrich F.
纳米孔传感是一项新兴的技术，具有许多生物传感应用，从使用生物孔进行DNA测序到使用固态孔进行生物分子分析。更稳定的固态纳米孔是生物传感应用的一个有吸引力的选择。尽管如此，生物分子与纳米孔表面的相互作用降低了纳米孔的稳定性，增加了使用成本。在这项研究中，我们研究了102个直径为11-18纳米的石英玻璃纳米孔的生物传感能力，这些纳米孔是用激光辅助毛细拉力制备的。纳米孔被组装成多个微流控芯片，重复使用长达19周。我们发现，使用真空存储和最少的洗涤步骤相结合，改善了纳米孔的使用周期。通过对用于检测短单链DNA寡核苷酸的DNA载体的定量分析，证明了重复使用周期中的单分子生物传感能力。保持低噪声和线性的电流-电压关系是生物传感的关键。在一个芯片中嵌入多个纳米孔可以进行90多种不同的测量，纳米孔可以每周重复使用19周以上。纳米孔的故障可归因于污染物的积累和溶解生物物理化学；生物技术；纳米技术；物理化学
Nanopore sensing is an emerging technology that has many biosensing applications ranging from DNA sequencing using biological pores to biomolecular analysis using solid-state pores. Solid-state nanopores that are more stable are an attractive choice for biosensing applications. Still, biomolecule interactions with the nanopore surface reduce nanopore stability and increase usage costs. In this study, we investigated the biosensing capability for 102 quartz glass nanopores with a diameter of 11–18 nm that were fabricated using laser-assisted capillary pulling. Nanopores were assembled into multiple microfluidic chips that were repeatedly used for up to 19 weeks. We find that using vacuum storage combined with minimal washing steps improved the number of use cycles for nanopores. The single-molecule biosensing capability over repeated use cycles was demonstrated by quantitative analysis of a DNA carrier designed for detection of short single-stranded DNA oligonucleotides. Maintaining low noise and linear current-voltage relation is crucial for biosensing Multiple nanopores embedded in a chip can make more than 90 different measurements Nanopores can be reused weekly over 19 weeks Nanopore failure can be attributed to contaminant accumulation and dissolution Biophysical chemistry; Biotechnology; Nanotechnology; Physical chemistry
登录
查看更多内容
影响因子：
16.6
作者：
Cai S;Pataillot-Meakin T;Shibakawa A;Ren R;Bevan CL;Ladame S;Ivanov AP;Edel JB
通讯作者：
Edel JB
影响因子：
17.1
作者：
Chou, Yung-Chien;Das, Paul Masih;Monos, Dimitri S.;Drndic, Marija
通讯作者：
Drndic, Marija
影响因子：
10.8
作者：
Chen, Kaikai;Zhu, Jinbo;Keyser, Ulrich F.
通讯作者：
Keyser, Ulrich F.
影响因子：
4.9
作者：
Kong, Jinglin;Zhu, Jinbo;Keyser, Ulrich F.
通讯作者：
Keyser, Ulrich F.
影响因子：
3.5
作者：
van den Hout, Michiel;Hall, Adam R.;Dekker, Nynke H.
通讯作者：
Dekker, Nynke H.