Susceptibility Testing of Bacteria Using Maldi-Tof Mass Spectrometry.

Susceptibility Testing of Bacteria Using Maldi-Tof Mass Spectrometry.
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DOI:
10.3389/fmicb.2018.01744
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发表时间:
2018
影响因子:
5.2
通讯作者:
Zimmermann S
Zimmermann S
中科院分区:
生物学2区
文献类型:
--
作者:
Burckhardt I;Zimmermann S

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基质辅助激光解吸电离飞行时间质谱法(MALDI-TOF MS)在十多年前被引入微生物常规。从那时起,它几乎取代了生物化学鉴定。它在准确性和成本方面是无与伦比的。从实验室的角度来看,这将是一个理想的方法,以取代传统的药敏试验,即Kirby-Baur琼脂扩散或测定最低抑制浓度(mic)。关于可能的药敏试验的首次报告已有10多年历史。然而,过去5年的发展是实质性的。除了一些例外,本审查的重点是在过去十年中取得的进展。
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was introduced into the microbiological routine more than 10 years ago. Since then it has almost replaced biochemical identification. It is unrivaled in terms of accuracy and cost. From a laboratory's perspective it would be an ideal method to replace classic susceptibility testing, that is Kirby-Baur agardiffusion or determination of minimal inhibitory concentrations (MICs). First reports on possible assays for susceptibility testing are more than 10 years old. However, the developments during the last 5 years were substantial. This review focuses with some exceptions on the progress, which was achieved during the last decade.
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