Survey on amacrine cells coupling to retrograde-identified ganglion cells in the mouse retina.
Survey on amacrine cells coupling to retrograde-identified ganglion cells in the mouse retina.
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对小鼠视网膜中无长突细胞与逆行识别的神经节细胞耦合的调查。
DOI：
10.1167/iovs.13-11774
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发表时间：
2013
影响因子：
4.4
通讯作者：
Wu,SamuelM
中科院分区：
文献类型：
--
作者：
Pang,Ji-Jie;Paul,DavidL;Wu,SamuelM
目的。视网膜无突细胞（ACs）可能在神经节细胞（GCs）上形成抑制性化学突触和潜在的兴奋性间隙连接。方法：采用先前建立的LY-NB （Lucifer yellow-Neurobiotin）逆行双标记技术，结合特异性抗体和共聚焦显微镜对GC和GC偶联的ACs进行鉴定。在野生型小鼠中，gc偶联ACs （nb阳性和ly阴性）占移位ACs的近11%，占常规ACs的4%，在Cx45 - / -和Cx36/45 - / -小鼠中分别占移位ACs的9%和4%。Cx36/45 - / -小鼠的胞体较小，但在其他菌株中是可变的。它们主要是γ-氨基丁酸（GABA）免疫反应（IR），位于GC层。它们仅占AC亚群的一小部分，包括野生型和ChAT转基因小鼠（ChAT- tdtomato）中的GABA-IR、甘氨酸- ir、钙黄素- ir、5- ht积累和on型胆碱乙酰转移酶（ChAT） ACs。在内丛状层（IPL）的远端80%，密集的GC树突与丰富的甘氨酸- ir和GABA-IR共存。在IPL内20%，稀疏的GC树突呈现主要的GABA带和稀疏的甘氨酸- ir。不同亚型的ACs可与gc偶联。具有相同免疫反应性的ACs可与GCs偶联或不偶联。除小型ac外，Cx36和Cx45在GC-AC耦合中占主导地位。GC-AC偶联的整体效力是中等的，特别是在近20%的IPL中，抑制化学信号以GABA ACs为主。
Purpose.: Retinal amacrine cells (ACs) may make inhibitory chemical synapses and potentially excitatory gap junctions on ganglion cells (GCs). The total number and subtypes of ACs coupled to the entire GC population were investigated in wild-type and three lines of transgenic mice.Methods.: GCs and GC-coupled ACs were identified by the previously established LY-NB (Lucifer yellow–Neurobiotin) retrograde double-labeling technique, in conjunction with specific antibodies and confocal microscopy.Results.: GC-coupled ACs (NB-positive and LY-negative) comprised nearly 11% of displaced ACs and 4% of conventional ACs in wild-type mice, and were 9% and 4% of displaced ACs in Cx45−/− and Cx36/45−/− mice, respectively. Their somas were small in Cx36/45−/− mice, but variable in other strains. They were mostly γ-aminobutyric acid (GABA)-immunoreactive (IR) and located in the GC layer. They comprised only a small portion in the AC subpopulations, including GABA-IR, glycine-IR, calretinin-IR, 5-HT-accumulating, and ON-type choline acetyltransferase (ChAT) ACs in wild-type and ChAT transgenic mice (ChAT-tdTomato). In the distal 80% of the inner plexiform layer (IPL), dense GC dendrites coexisted with rich glycine-IR and GABA-IR. In the inner 20% of the IPL, sparse GC dendrites presented with a major GABA band and sparse glycine-IR.Conclusions.: Various subtypes of ACs may couple to GCs. ACs of the same immunoreactivity may either couple or not couple to GCs. Cx36 and Cx45 dominate GC-AC coupling except for small ACs. The overall potency of GC-AC coupling is moderate, especially in the proximal 20% of the IPL, where inhibitory chemical signals are dominated by GABA ACs.
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兔子视网膜中一种开关无长突细胞的光反应。
DOI：
10.1152/jn.1995.74.6.2460
发表时间：
1995
期刊：
Journal of neurophysiology.
影响因子：
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作者：
Dacheux,RF;Raviola,E
通讯作者：
Raviola,E
暗适应兔视网膜长程轴突无长突细胞的反应特性
影响因子：
1.9
作者：
W. Taylor
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W. Taylor
三种软骨鱼和一条枝条鱼（Polypterus）视网膜中钙结合蛋白和钙结合蛋白 D-28K 表达的免疫细胞化学研究
影响因子：
3.8
作者：
Patricia Graña;R. Anadón;J. Yáñez
通讯作者：
J. Yáñez
影响因子：
2.5
作者：
Ghosh, KK;Bujan, S;Wässle, H
通讯作者：
Wässle, H
神经递质通过视网膜间隙连接耦合
DOI：
10.1523/jneurosci.18-24-10594.1998
发表时间：
1998
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
D. I. Vaney;J. Charles Nelson;D. Pow
通讯作者：
D. Pow