Achyranthes bidentata polypeptides reduces oxidative stress and exerts protective effects against myocardial ischemic/reperfusion injury in rats.
Achyranthes bidentata polypeptides reduces oxidative stress and exerts protective effects against myocardial ischemic/reperfusion injury in rats.
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牛膝多肽降低氧化应激对大鼠心肌缺血/再灌注损伤的保护作用
DOI：
10.3390/ijms141019792
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发表时间：
2013-09-30
影响因子：
5.6
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Tie R;Ji L;Nan Y;Wang W;Liang X;Tian F;Xing W;Zhu M;Li R;Zhang H
牛膝是一种中草药，据报道具有神经保护作用。然而，其在心脏保护中的作用在很大程度上仍然未知。本研究旨在观察牛膝多肽（Achyranthes bidentata polypeptides，ABPP）预处理对大鼠心肌缺血/再灌注（MI/R）损伤的影响，并探讨其可能的机制。用ABPP（10 mg/kg/d，i.p.）或生理盐水，每天一次，持续一周。随后，所有动物均进行30 min心肌缺血，随后再灌注4 h。ABPP预处理1周可明显改善MI/R后心功能。同时，ABPP可减少再灌注后心肌梗死面积，降低血浆肌酸激酶（CK）/乳酸脱氢酶（LDH）活性，减少心肌细胞凋亡。ABPP预处理可显著抑制I/R心肌超氧化物歧化酶活性、gp 91 phox表达、丙二醛生成。此外，ABPP处理抑制I/R大鼠心脏中PTEN表达并增加Akt磷酸化。PI 3 K抑制剂wortmannin可阻断Akt的激活，并消除ABPP刺激的抗氧化作用和心脏保护作用。我们的研究首次证明，ABPP降低氧化应激，并对大鼠心肌梗死/再灌注损伤发挥心脏保护作用。抑制PTEN和激活Akt可能有助于ABPP的抗氧化能力和心脏保护作用。
Achyranthes bidentata, a Chinese medicinal herb, is reported to be neuroprotective. However, its role in cardioprotection remains largely unknown. Our present study aimed to investigate the effects of Achyranthes bidentata polypeptides (ABPP) preconditioning on myocardial ischemia/reperfusion (MI/R) injury and to test the possible mechanisms. Rats were treated with ABPP (10 mg/kg/d, i.p.) or saline once daily for one week. Afterward, all the animals were subjected to 30 min of myocardial ischemia followed by 4 h of reperfusion. ABPP preconditioning for one week significantly improved cardiac function following MI/R. Meanwhile, ABPP reduced infarct size, plasma creatine kinase (CK)/lactate dehydrogenase (LDH) activities and myocardial apoptosis at the end of reperfusion in rat hearts. Moreover, ABPP preconditioning significantly inhibited superoxide generation, gp91phox expression, malonaldialdehyde formation and enhanced superoxide dismutase activity in I/R hearts. Furthermore, ABPP treatment inhibited PTEN expression and increased Akt phosphorylation in I/R rat heart. PI3K inhibitor wortmannin blocked Akt activation, and abolished ABPP-stimulated anti-oxidant effect and cardioprotection. Our study demonstrated for the first time that ABPP reduces oxidative stress and exerts cardioprotection against MI/R injury in rats. Inhibition of PTEN and activation of Akt may contribute to the anti-oxidant capacity and cardioprotection of ABPP.
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