Development of the liver in the chicken embryo. I. Hepatic cords and sinusoids
Development of the liver in the chicken embryo. I. Hepatic cords and sinusoids
复制标题
鸡胚肝脏的发育。
DOI：
10.1002/ar.1092340411
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
M. J. Cavey
中科院分区：
文献类型：
--
作者：
G. K. Wong;M. J. Cavey
鸡胚肝脏中的造血在孵育的第7天开始（Hamburger和汉密尔顿阶段30），并在第14天达到峰值（阶段40）。在此时间范围内，肝细胞的分化进行了检查，通过光学显微镜，透射和扫描电子显微镜，和形态计量学。禽类的肝脏是一个由树枝状索和不连续的血窦组成的紧密堆积的团块。肝细胞呈金字塔形，环绕着穿过索中心的胆小管。半薄切片（通过浸润和包埋在乙二醇甲基丙烯酸酯中制成）用苏木精和伊红染色，以评估器官的一般结构和肝细胞的脂质含量，并通过高碘酸-希夫反应和苏木精观察糖原的细胞质储存。在早期造血中，具有可证实的糖原的肝细胞的数量不规则地波动，并且随着造血攀升至峰值，含糖原细胞的比例逐渐增加。大多数分化中的肝细胞直到第39和40阶段才缺乏脂滴。从第30阶段到第35阶段，肝细胞体积福尔斯降至其最低值。随后（第36至40阶段），细胞体积增加，肝细胞达到相对均匀的大小。分化中的肝细胞的超微结构变化，包括改变线粒体，内质网，高尔基体，记录。这些关于前肝细胞群和肝血管系统的形态学和形态测量学发现涵盖了许多脊椎动物中被认为对肝造血至关重要的3种元素中的2种。© Willey利斯，Inc.
Hemopoiesis in the liver of the chicken embryo begins on day 7 of incubation (Hamburger and Hamilton Stage 30) and peaks on day 14 (Stage 40). During this time frame, the differentiation of hepatic cells was examined by light microscopy, transmission and scanning electron microscopy, and morphometry. The avian liver is a closely packed mass of dendriform cords and discontinuous sinusoids. Hepatocytes are pyramidal in shape, and they ring the bile canaliculi which run through the centers of the cords. Semithin sections, made possible by infiltration and embedding in glycol methacrylate, were stained with hematoxylin and eosin to assess the general architecture of the organ and the lipid content of the hepatocytes and by the periodic acid‐Schiff reaction and hematoxylin to visualize the cytoplasmic stores of glycogen. The number of hepatocytes with demonstrable glycogen fluctuates erratically in early hemopoiesis, and the proportion of glycogen‐containing cells progressively increases as hemopoiesis climbs to a peak. Most differentiating hepatocytes are devoid of lipid droplets until Stages 39 and 40. From Stage 30 to 35, hepatocyte volume falls to its lowest value. Subsequently (Stages 36 to 40), cell volume increases and hepatocytes achieve a relatively uniform size. Ultrastructural changes in the differentiating hepatocytes, including alterations to the mitochondria, endoplasmic reticulum, and Golgi apparatus, are documented. These morphological and morphometric findings on the prehepatocyte population and hepatic vasculature cover 2 of the 3 elements deemed critical to hepatic hemopoiesis in many vertebrates.© Willey‐Liss, Inc.
影响因子：
--
作者：
BANKSTON, PW;PINO, RM
通讯作者：
PINO, RM
影响因子：
56.9
作者：
ROTHMAN, JE
通讯作者：
ROTHMAN, JE