Group 1 and group 2 hemagglutinin stalk antibody response according to age.
Group 1 and group 2 hemagglutinin stalk antibody response according to age.
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DOI：
10.3389/fimmu.2023.1194073
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发表时间：
2023
影响因子：
7.3
通讯作者：
Aydillo, Teresa
中科院分区：
文献类型：
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作者：
Sanchez-de Prada, Laura;Sanz-Munoz, Ivan;Sun, Weina;Palese, Peter;de Lejarazu, Raul Ortiz;Eiros, Jose Maria;Garcia-Sastre, Adolfo;Aydillo, Teresa
季节性流感疫苗引发的抗体主要针对血凝素（HA）的头部。然而，针对茎结构域的抗体具有交叉反应性，并已被证明在降低流感疾病严重程度方面发挥作用。考虑到队列的年龄，我们调查了季节性流感疫苗接种后HA茎特异性抗体的诱导。在2018年流感疫苗运动（IVC）期间共招募了166名个体，并将其分为年龄<50 （n = 14）、50 - 64 （n = 34）、65-79 （n = 61）和≥80 （n = 57）组。在第0天和第28天，分别用含有来自野生鸟类的HA头部结构域（H6或H14）和来自人类H1或H3的茎结构域的重组病毒（cH6/1和cH14/3），用ELISA法定量检测茎秆特异性抗体。计算几何平均滴度（GMT）和翻倍上升（GMFR），并使用经错误发现率（FDR）和Wilcoxon检验（p <0.05）校正的方差分析评估差异。除≥80岁年龄组外，所有年龄组接种流感疫苗后抗茎突抗体均有一定程度的增加。此外，小于65岁的接种者在接种前后的第1组抗体滴度高于第2组。同样，与老年人（≥80岁）相比，<50岁组的疫苗接种者抗秸秆抗体滴度增加更高，特别是1组抗秸秆抗体。季节性流感疫苗可诱导针对1组和2组ha的交叉反应性抗茎抗体。然而，在老年组中观察到低应答，突出了免疫衰老对充分的体液免疫应答的影响。
Antibodies elicited by seasonal influenza vaccines mainly target the head of hemagglutinin (HA). However, antibodies against the stalk domain are cross-reactive and have been proven to play a role in reducing influenza disease severity. We investigated the induction of HA stalk-specific antibodies after seasonal influenza vaccination, considering the age of the cohorts. A total of 166 individuals were recruited during the 2018 influenza vaccine campaign (IVC) and divided into groups: <50 (n = 14), 50–64 (n = 34), 65–79 (n = 61), and ≥80 (n = 57) years old. Stalk-specific antibodies were quantified by ELISA at day 0 and day 28 using recombinant viruses (cH6/1 and cH14/3) containing an HA head domain (H6 or H14) from wild bird origin with a stalk domain from human H1 or H3, respectively. The geometric mean titer (GMT) and the fold rise (GMFR) were calculated, and differences were assessed using ANOVA adjusted by the false discovery rate (FDR) and the Wilcoxon tests (p <0.05). All age groups elicited some level of increase in anti-stalk antibodies after receiving the influenza vaccine, except for the ≥80-year-old cohort. Additionally, <65-year-old vaccinees had higher group 1 antibody titers versus group 2 before and after vaccination. Similarly, vaccinees within the <50-year-old group showed a higher increase in anti-stalk antibody titers when compared to older individuals (≥80 years old), especially for group 1 anti-stalk antibodies. Seasonal influenza vaccines can the induction of cross-reactive anti-stalk antibodies against group 1 and group 2 HAs. However, low responses were observed in older groups, highlighting the impact of immunosenescence in adequate humoral immune responses.
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影响因子：
30.5
作者：
Nachbagauer R;Choi A;Hirsh A;Margine I;Iida S;Barrera A;Ferres M;Albrecht RA;García-Sastre A;Bouvier NM;Ito K;Medina RA;Palese P;Krammer F
通讯作者：
Krammer F
影响因子：
3.7
作者：
Krammer F;Margine I;Tan GS;Pica N;Krause JC;Palese P
通讯作者：
Palese P
影响因子：
3.8
作者：
Madsen, Anders;Azimi, Linda;Jul-Larsen, Asne
通讯作者：
Jul-Larsen, Asne
影响因子：
82.9
作者：
Nachbagauer, Raffael;Feser, Jodi;Krammer, Florian
通讯作者：
Krammer, Florian
影响因子：
9.2
作者：
通讯作者：
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