High-frequency stimulation of nucleus accumbens changes in dopaminergic reward circuit.
High-frequency stimulation of nucleus accumbens changes in dopaminergic reward circuit.
复制标题
伏隔核的高频刺激会改变多巴胺能奖励回路。
DOI：
10.1371/journal.pone.0079318
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Yan N;Chen N;Zhu H;Zhang J;Sim M;Ma Y;Wang W
脑深部电刺激（DBS）是一种潜在的药物渴求和复发的治疗方法，但其机制知之甚少。我们研究了高频刺激（HFS）吗啡诱导大鼠单侧NAc时神经递质水平的变化。将60只成年Wistar大鼠随机分为5组：对照组（给予生理盐水），仅吗啡组（全身给予吗啡，无电极植入），吗啡假刺激组（植入电极系统给予吗啡，但不给予刺激），吗啡刺激组（系统给予吗啡并植入电极和刺激）和生理盐水刺激组（给予生理盐水并植入电极和刺激）。将刺激电极立体定位植入单侧NAc的核心，并将微透析探针单侧降低至同侧腹侧被盖区（VTA）、NAc和腹侧苍白球（VP）。通过高效液相色谱法（HPLC）分析同侧VTA、NAc和VP中微透析探针的样品中的谷氨酸（Glu）和γ-氨基丁酸（GABA）。与对照组相比，吗啡组大鼠同侧NAc和VP的Glu水平升高，而同侧VTA的Glu水平无明显变化。与对照组相比，吗啡组大鼠同侧NAc、VP和VTA的GABA含量显著降低。在吗啡诱导的大鼠中Glu增加和GABA减少的概况表明，在这些脑区中存在兴奋性神经传递增加。与单纯吗啡组相比，吗啡刺激组同侧VTA、NAc和VP中Glu含量显著降低，GABA含量显著升高。吗啡-假刺激组与单纯吗啡组相比无显著变化。这些结果表明，单侧NAc刺激抑制吗啡诱导的大鼠中皮质边缘奖赏回路兴奋性神经传递的过度激活。
Deep brain stimulation (DBS) of the nucleus accumbens (NAc) is a potential remedial therapy for drug craving and relapse, but the mechanism is poorly understood. We investigated changes in neurotransmitter levels during high frequency stimulation (HFS) of the unilateral NAc on morphine-induced rats. Sixty adult Wistar rats were randomized into five groups: the control group (administration of saline), the morphine-only group (systematic administration of morphine without electrode implantation), the morphine-sham-stimulation group (systematic administration of morphine with electrode implantation but not given stimulation), the morphine-stimulation group (systematic administration of morphine with electrode implantation and stimulation) and the saline-stimulation group (administration of saline with electrode implantation and stimulation). The stimulation electrode was stereotaxically implanted into the core of unilateral NAc and microdialysis probes were unilaterally lowered into the ipsilateral ventral tegmental area (VTA), NAc, and ventral pallidum (VP). Samples from microdialysis probes in the ipsilateral VTA, NAc, and VP were analyzed for glutamate (Glu) and γ-aminobutyric acid (GABA) by high-performance liquid chromatography (HPLC). The levels of Glu were increased in the ipsilateral NAc and VP of morphine-only group versus control group, whereas Glu levels were not significantly changed in the ipsilateral VTA. Furthermore, the levels of GABA decreased significantly in the ipsilateral NAc, VP, and VTA of morphine-only group when compared with control group. The profiles of increased Glu and reduced GABA in morphine-induced rats suggest that the presence of increased excitatory neurotransmission in these brain regions. The concentrations of the Glu significantly decreased while the levels of GABA increased in ipsilateral VTA, NAc, and VP in the morphine-stimulation group compared with the morphine-only group. No significant changes were seen in the morphine-sham stimulation group compared with the morphine-only group. These findings indicated that unilateral NAc stimulation inhibits the morphine-induced rats associated hyperactivation of excitatory neurotransmission in the mesocorticolimbic reward circuit.
登录
查看更多内容
吗啡降低前扣带皮层细胞外谷氨酸水平：自由活动大鼠的体内微透析研究
影响因子：
2.9
作者：
Hao, Y;Yang, JY;Wu, MF
通讯作者：
Wu, MF
影响因子：
3.4
作者：
Caillé, S;Parsons, LH
通讯作者：
Parsons, LH
影响因子：
1.7
作者：
Gao, GD;Wang, XL;Li, AN
通讯作者：
Li, AN
影响因子：
3
作者：
Hiller, A.;Loeffler, S.;Moser, A.
通讯作者：
Moser, A.
影响因子：
5.3
作者：
Cornish, JL;Kalivas, PW
通讯作者：
Kalivas, PW