14-3-3s function in plant cadmium response by changes of glutathione and glutathione synthesis in Arabidopsis
14-3-3s function in plant cadmium response by changes of glutathione and glutathione synthesis in Arabidopsis
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14-3-3s 在拟南芥中谷胱甘肽和谷胱甘肽合成变化对植物镉响应中的作用
DOI：
10.1016/j.envexpbot.2019.04.009
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发表时间：
2019-07
影响因子：
5.7
通讯作者：
Fu Zheng Wei
中科院分区：
文献类型：
--
作者：
Zhang Bing Lei;Guo Chen Chen;Ding Feng;Lu Ying Tang;Fu Zheng Wei
众所周知，植物对镉这种广泛存在的土壤污染物的反应是通过积累谷胱甘肽（GSH）和植物螯合素（PC）。然而，调节GSH和PC合成的因子仍有待进一步确定。本研究发现，14-3-3κ和λ对植物Cd耐受性具有负调控作用，因为Cd积累量较高的双突变体14-3-3κλ对Cd耐受性增强，而过表达14-3-3κ或14-3-3κλ导致Cd胁迫下主根伸长和鲜重变化的Cd敏感性增加。与胁迫野生型植株相比，14-3-3 - κλ突变体诱导GSH和PC合成基因的表达以及GSH和PC积累的水平进一步提高，而14-3-3 - κ或14-3-3 - λ过表达系的GSH和PC积累水平降低，表明GSH和PC积累参与了14-3-3 - s介导的植株Cd反应。我们的观察结果进一步支持了这一观点，即与14-3-3 - κλ幼苗相比，同时处理Cd和丁硫氨酸亚砜胺（GSH合成抑制剂）的幼苗对Cd的耐受性降低。此外，cd诱导的ROS积累在14-3-3 - κλ植株中受到抑制，而在35s::14-3-3 - κ和35s::14-3-3 - λ转基因植株中与野生型植株相比，cd诱导的ROS积累在35s::14-3-3 - κ和35s::14-3-3 - λ转基因植株中进一步增强，与GSH积累一致。综上所述，14-3-3 s通过改变拟南芥的谷胱甘肽和谷胱甘肽合成来参与植物对Cd的响应。
It is well-known that plants respond to the toxin cadmium (Cd), a widespread soil contaminant, by accumulating glutathione (GSH) and phytochelatin (PC). However, the factor(s) in the regulation of GSH and PC synthesis remain to be further identified. Here, we report that 14-3-3 κ and λ negatively regulate plant Cd tolerance because the double mutant14-3-3κλwith higher Cd accumulation exhibited enhanced Cd tolerance, while overexpressing14-3-3κor14-3-3λresulted in increased Cd sensitivity in terms of changes of primary root elongation and fresh weight under Cd stress. The expression induction of the genes for GSH and PC synthesis and thus GSH and PC accumulation were further promoted in the14-3-3κλmutant but reduced in14-3-3κor14-3-3λoverexpression lines compared with those in the stressed wild-type plants, suggesting the involvement of GSH and PC accumulation in 14-3-3s-mediated plant Cd response. This note was further supported by our observation that higher Cd tolerance was compromised in14-3-3κλseedlings treated simultaneously with Cd and buthionine sulfoximine, an inhibitor of GSH synthesis, compared with that in14-3-3κλseedlings treated with Cd alone. In addition, Cd-induced ROS accumulation was suppressed in14-3-3κλplants but further enhanced in35S::14-3-3κand35S::14-3-3λtransgenic lines compared with that in wild-type plants, in consistent with GSH accumulations in these plants. Together, 14-3-3 s function in plant Cd response by changes of glutathione and glutathione synthesis in Arabidopsis.
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影响因子：
7.4
作者：
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通讯作者：
Cobbett, CS
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Cardarelli M