Identification of differentially accumulated proteins involved in regulating independent and combined osmosis and cadmium stress response in Brachypodium seedling roots.
Identification of differentially accumulated proteins involved in regulating independent and combined osmosis and cadmium stress response in Brachypodium seedling roots.
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DOI：
10.1038/s41598-018-25959-8
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发表时间：
2018-05-17
影响因子：
4.6
通讯作者：
Yan Y
中科院分区：
文献类型：
--
作者：
Chen Z;Zhu D;Wu J;Cheng Z;Yan X;Deng X;Yan Y
在这项研究中，我们的目的是确定差异积累的蛋白质（DAP）参与PEG模拟渗透胁迫，镉（Cd 2+）胁迫，以及它们的组合胁迫反应在短柄草幼苗根。结果表明，PEG和Cd 2+复合胁迫对短柄草幼苗根系生长、生理特性和超微结构的影响均较单一胁迫显著。共鉴定出106个DAP，它们对根中的单独和组合应力有反应。这些DAP主要参与能量代谢、解毒和应激防御以及蛋白质代谢。主成分分析显示，Cd 2+胁迫和复合胁迫处理的DAP比渗透胁迫处理的DAP更接近，表明Cd 2+胁迫和复合胁迫对根系蛋白质组的影响比单独的渗透胁迫更严重。蛋白质-蛋白质相互作用分析突出了14-3-3为中心的子网络，协同响应渗透压和Cd 2+胁迫及其组合应力。14个关键DAP基因的定量实时聚合酶链反应（qRT-PCR）分析显示，大多数基因的转录和翻译表达模式之间的一致性。提出了不同胁迫条件下短柄草幼苗根系蛋白质组代谢变化的可能途径，揭示了植物根系对逆境的复杂协同响应网络。
In this study, we aimed to identify differentially accumulated proteins (DAPs) involved in PEG mock osmotic stress, cadmium (Cd2+) stress, and their combined stress responses in Brachypodium distachyon seedling roots. The results showed that combined PEG and Cd2+ stresses had more significant effects on Brachypodium seedling root growth, physiological traits, and ultrastructures when compared with each individual stress. Totally, 106 DAPs were identified that are responsive to individual and combined stresses in roots. These DAPs were mainly involved in energy metabolism, detoxification and stress defense and protein metabolism. Principal component analysis revealed that DAPs from Cd2+ and combined stress treatments were grouped closer than those from osmotic stress treatment, indicating that Cd2+ and combined stresses had more severe influences on the root proteome than osmotic stress alone. Protein–protein interaction analyses highlighted a 14-3-3 centered sub-network that synergistically responded to osmotic and Cd2+ stresses and their combined stresses. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 14 key DAP genes revealed that most genes showed consistency between transcriptional and translational expression patterns. A putative pathway of proteome metabolic changes in Brachypodium seedling roots under different stresses was proposed, which revealed a complicated synergetic responsive network of plant roots to adverse environments.
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