Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts.
Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts.
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DOI：
10.1371/journal.pone.0080720
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Firth AE
中科院分区：
文献类型：
--
作者：
Cook S;Chung BY;Bass D;Moureau G;Tang S;McAlister E;Culverwell CL;Glücksman E;Wang H;Brown TD;Gould EA;Harbach RE;de Lamballerie X;Firth AE
我们研究了从野外采集的蚊子和摇蚊（双翅目）中测序的小 RNA (sRNA) 是否可以用作一系列物种和病毒组内病毒流行的代理特征，使用从野生捕获样本中测序的 sRNA 来为特别感兴趣的样本的总 RNA 深度测序提供信息。使用这种策略，我们对成年按蚊 (Anopheles maculipennis s.l.) 进行了测序。蚊子的一种先前未描述的与慢性蜜蜂麻痹病毒相关的病毒的明显几乎完整的基因组，并且来自 Ochlerotatus caspius 和 Oc.碎屑蚊子，几乎完整的昆虫病毒基因组。我们还重建了与至少九种其他病毒相关的长序列（1503-6557 nt）。至关重要的是，检测到的几个序列是从宿主生物体中重建的，与之前分离或发现相关病毒的宿主生物体高度不同。显然，自然界中的病毒传播和维持周期可能比之前预期的更加复杂和分类多样化。
We investigated whether small RNA (sRNA) sequenced from field-collected mosquitoes and chironomids (Diptera) can be used as a proxy signature of viral prevalence within a range of species and viral groups, using sRNAs sequenced from wild-caught specimens, to inform total RNA deep sequencing of samples of particular interest. Using this strategy, we sequenced from adult Anopheles maculipennis s.l. mosquitoes the apparently nearly complete genome of one previously undescribed virus related to chronic bee paralysis virus, and, from a pool of Ochlerotatus caspius and Oc. detritus mosquitoes, a nearly complete entomobirnavirus genome. We also reconstructed long sequences (1503-6557 nt) related to at least nine other viruses. Crucially, several of the sequences detected were reconstructed from host organisms highly divergent from those in which related viruses have been previously isolated or discovered. It is clear that viral transmission and maintenance cycles in nature are likely to be significantly more complex and taxonomically diverse than previously expected.
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影响因子：
5.8
作者：
Firth AE;Jagger BW;Wise HM;Nelson CC;Parsawar K;Wills NM;Napthine S;Taubenberger JK;Digard P;Atkins JF
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DOI：
10.4269/ajtmh.2009.80.85
发表时间：
2009-01-01
影响因子：
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作者：
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作者：
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