Interleukin-17 Contributes to Chikungunya Virus-Induced Disease.
Interleukin-17 Contributes to Chikungunya Virus-Induced Disease.
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DOI：
10.1128/mbio.00289-22
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发表时间：
2022-04-26
期刊：
影响因子：
6.4
通讯作者：
Mahalingam, Suresh
中科院分区：
文献类型：
--
作者：
Liu, Xiang;Poo, Yee-Suan;Alves, Juliana C.;Almeida, Roque P.;Mostafavi, Helen;Tang, Patrick Chun Hean;Bucala, Richard;Teixeira, Mauro M.;Taylor, Adam;Zaid, Ali;Mahalingam, Suresh
由蚊媒虫媒病毒如基孔肯雅病毒（CHIKV）引起的甲病毒性关节炎在最初的急性疾病后可持续数月。在这里，我们研究了白细胞介素-17（IL-17），一种参与慢性自身免疫性关节病（如类风湿性关节炎）的细胞因子，对甲病毒性关节病发展的贡献。来自表现出急性和慢性疾病的CHIKV感染患者的血清显示高水平的IL-17、IL-6、IL-21、IL-22和IL-23，尤其是在疾病的慢性期。我们试图使用CHIKV感染和疾病的小鼠模型，使用野生型和IL-17 A缺陷型小鼠来验证这些发现。用CHIKV感染小鼠，并在指定时间点收获关节和肌肉组织。采用免疫组化法检测组织浸润情况，并评估组织中细胞因子的mRNA和蛋白表达。使用组织学评估关节和肌肉病理学。与野生型小鼠相比，缺乏IL-17 A的CHIKV感染小鼠显示出减少的组织炎症和中性粒细胞浸润。这些研究显示IL-17在CHIKV感染的急性期以及急性后疾病消退期中的作用。
Alphaviral arthritides caused by mosquito-borne arboviruses such as chikungunya virus (CHIKV) can persist for months after the initial acute disease. Here, we investigated the contribution of interleukin-17 (IL-17), a cytokine involved in chronic autoimmune arthropathies such as rheumatoid arthritis, to the development of alphaviral arthropathy. Sera from CHIKV-infected patients who displayed both acute and chronic disease showed high levels of IL-17, IL-6, IL-21, IL-22, and IL-23, especially during the chronic phase of disease. We sought to validate these findings using a mouse model of CHIKV infection and disease using wild-type and IL-17A-deficient mice. Mice were infected with CHIKV, and joint and muscle tissues were harvested at designated time points. Tissue infiltrates were examined by immunohistochemistry, and tissue mRNA and protein expression of cytokines was assessed. Joint and muscle pathology was assessed using histology. CHIKV-infected mice lacking IL-17A showed reduced tissue inflammation and neutrophil infiltration, compared to wild-type mice. These investigations showed a role for IL-17 in the acute phase of CHIKV infection and also during the postacute disease resolution phase.
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DOI：
10.1093/infdis/jiq042
发表时间：
2011-01-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Chow A;Her Z;Ong EK;Chen JM;Dimatatac F;Kwek DJ;Barkham T;Yang H;Rénia L;Leo YS;Ng LF
通讯作者：
Ng LF
影响因子：
4.6
作者：
Bankhead P;Loughrey MB;Fernández JA;Dombrowski Y;McArt DG;Dunne PD;McQuaid S;Gray RT;Murray LJ;Coleman HG;James JA;Salto-Tellez M;Hamilton PW
通讯作者：
Hamilton PW
影响因子：
4.4
作者：
Sheel, Meru;Beattie, Lynette;Engwerda, Christian R.
通讯作者：
Engwerda, Christian R.
影响因子：
3.7
作者：
Katayama M;Ohmura K;Yukawa N;Terao C;Hashimoto M;Yoshifuji H;Kawabata D;Fujii T;Iwakura Y;Mimori T
通讯作者：
Mimori T
影响因子：
3.7
作者：
Won HY;Lee JA;Park ZS;Song JS;Kim HY;Jang SM;Yoo SE;Rhee Y;Hwang ES;Bae MA
通讯作者：
Bae MA