Enumeration of the colony-forming units-fibroblast from mouse and human bone marrow in normal and pathological conditions.
Enumeration of the colony-forming units-fibroblast from mouse and human bone marrow in normal and pathological conditions.
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DOI：
10.1016/j.scr.2008.07.007
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发表时间：
2009-01
影响因子：
1.2
通讯作者：
Robey, Pamela G.
中科院分区：
文献类型：
--
作者：
Kuznetsov, Sergei A.;Mankani, Mahesh H.;Bianco, Paolo;Robey, Pamela G.
骨髓基质细胞群，包含多潜能骨骼干细胞的一个子集，越来越多地被考虑用于组织工程和干细胞治疗，然而它们在骨骼疾病的发病机制中的作用却很少被认识到。我们比较了正常和病理间质克隆生成细胞，集落形成单位-成纤维细胞（CFU-Fs）的浓度。最初，通过证明胎牛血清热灭活能显著抑制菌落形成，优化了培养条件。使用非热灭活胎牛血清，5个近交系小鼠的集落形成效率（CFE）为每1 × 105个有核细胞3.5±1.0 ~ 11.5±4.0。在四种骨骼深度受累的转基因系中，与野生型幼崽相比，CFE要么显著减少，要么显著增加。在正常供体中，CFE随年龄略有下降，儿童平均为52.2±4.1，成人平均为32.3±3.0。CFE在几种骨骼、代谢和血液学疾病患者中显著改变：先天性全身性脂肪营养不良、软骨发育不全（SADDAN）、假性软骨发育不全和骨Paget病患者中减少，在高尿酸血症和镰状细胞性贫血患者中升高。我们的研究结果表明，在适当的培养条件下，CFE值可以为骨/骨髓病理生理学提供有用的见解。
Bone marrow stromal cell populations, containing a subset of multipotential skeletal stem cells, are increasingly contemplated for use in tissue engineering and stem cell therapy, whereas their involvement in the pathogenetic mechanisms of skeletal disorders is far less recognized. We compared the concentrations of stromal clonogenic cells, colony forming units–fibroblast (CFU-Fs), in norm and pathology. Initially, culture conditions were optimized by demonstrating that fetal bovine serum heat inactivation could significantly repress colony formation. Using non-heat-inactivated fetal bovine serum, the concentration of CFU-Fs (colony-forming efficiency, CFE) ranged from 3.5 ± 1.0 to 11.5 ± 4.0 per 1 × 105 nucleated cells in five inbred mouse strains. In four transgenic lines with profound bone involvement, CFE was either significantly reduced or increased compared to wild-type littermates. In normal human donors, CFE decreased slightly with age and averaged 52.2 ± 4.1 for children and 32.3 ± 3.0 for adults. CFE was significantly altered in patients with several skeletal, metabolic, and hematological disorders: reduced in congenital generalized lipodystrophy, achondroplasia (SADDAN), pseudoachondroplasia, and Paget disease of bone and elevated in alcaptonuria and sickle cell anemia. Our findings indicate that under appropriate culture conditions, CFE values may provide useful insights into bone/bone marrow pathophysiology.
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影响因子：
4.1
作者：
Akintoye, Sunday O.;Lam, Thanh;Robey, Pamela G.
通讯作者：
Robey, Pamela G.
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通讯作者：
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影响因子：
6.2
作者：
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通讯作者：
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