Functionalization of iron oxide nanoparticles with a versatile epoxy amine linker.
Functionalization of iron oxide nanoparticles with a versatile epoxy amine linker.
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DOI：
10.1039/c0jm00808g
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发表时间：
2010
影响因子：
--
通讯作者：
Pham W
中科院分区：
文献类型：
--
作者：
Nickels M;Xie J;Cobb J;Gore JC;Pham W
已经合成了由末端环氧化物和末端胺两者组成的合成多样性连接体分子，并且显示出具有期望的反应性。原理证明实验表明，所制备的接头分子具有对葡聚糖涂覆的铁纳米颗粒具有反应性的能力，基本上以平均每个纳米颗粒50个接头的效率将表面醇转化为胺。一旦纳米颗粒的表面已经被官能化，随后用叶酸和异硫氰酸荧光素两者官能化铁纳米颗粒，平均效率分别为每个纳米颗粒20和3个分子。然后将标记的纳米颗粒与叶酸受体阳性和阴性细胞系一起孵育，其显示颗粒在受体阳性细胞系中的优先积累。除了基于荧光的测定之外，使用T2加权MRI成像证明纳米颗粒的积累，其显示纳米颗粒的铁核存在于所需的细胞系内。总的来说，这种连接体已经显示出使纳米颗粒表面官能化的能力，并且理论上可以用于标记用于体内治疗或诊断的多种其他靶向剂或成像剂。
A synthetically diverse linker molecule consisting of both a terminal epoxide and a terminal amine has been synthesized and shown to have the desired reactivity. Proof of principle experimentation showed that the prepared linker molecule possessed the ability to be reactive towards dextran coated iron nanoparticles, essentially converting the surface alcohols to amines with an efficiency on average of 50 linkers per nanoparticle. Once the surface of the nanoparticles had been functionalized, the iron nanoparticles were subsequently functionalized with both folic acid and fluorescein isothiocyanate, with an average efficiency of 20 and 3 molecules per nanoparticle, respectively. The labeled nanoparticles were then incubated with both folate receptor positive and negative cell lines, which showed a preferential accumulation of the particles in the receptor positive cell line. In addition to the fluorescence based assays, accumulation of the nanoparticles was demonstrated using T2-weighted MRI imaging, which showed that the iron core of the nanoparticle was present within the desired cell line. Overall, this linker has shown the ability to functionalize the surface of nanoparticles and can theoretically be used to label a wide variety of other targeting agents or imaging agents for in vivo therapies or diagnostics.
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