A multi-structural single cell model of force-induced interactions of cytoskeletal components.
A multi-structural single cell model of force-induced interactions of cytoskeletal components.
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DOI：
10.1016/j.biomaterials.2013.04.022
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发表时间：
2013-08
期刊：
影响因子：
14
通讯作者：
Lacroix D
中科院分区：
文献类型：
--
作者：
Barreto S;Clausen CH;Perrault CM;Fletcher DA;Lacroix D
基于实验技术和理论的细胞骨架力学计算模型已被提出。张拉整体性是一种重要的力产生模型，但它无法预测单个CSK组件的力学性能，也无法解释与细胞骨架破坏剂相结合的不同单细胞刺激技术研究的差异。提出了一种新的数值概念，定义了一个多结构的三维有限元（FE）模型的单贴壁细胞的生物物理和生物化学差异的力学作用下的每个细胞骨架组件的负载。该模型包括在细胞质和细胞核内的被肌动蛋白皮质包围的预应力肌动蛋白束和微管。我们进行了原子力显微镜（AFM）实验的数值模拟细胞模型进行压缩负荷。CSK组分的数值作用与暴露于不同细胞分裂素破坏药物的U2 OS-骨肉瘤细胞和NIH-3 T3成纤维细胞上的AFM力测量相证实。计算模拟表明，肌动蛋白皮层和微管是抗压缩的主要靶点。这是一种新的数值工具，它解释了皮层的特定作用，并克服了将该成分与体外其他网络分离的困难。这说明，细胞骨架结构与它们自身的性质相结合，对于细胞力学的完整描述是必要的。
Several computational models based on experimental techniques and theories have been proposed to describe cytoskeleton (CSK) mechanics. Tensegrity is a prominent model for force generation, but it cannot predict mechanics of individual CSK components, nor explain the discrepancies from the different single cell stimulating techniques studies combined with cytoskeleton-disruptors. A new numerical concept that defines a multi-structural 3D finite element (FE) model of a single-adherent cell is proposed to investigate the biophysical and biochemical differences of the mechanical role of each cytoskeleton component under loading. The model includes prestressed actin bundles and microtubule within cytoplasm and nucleus surrounded by the actin cortex. We performed numerical simulations of atomic force microscopy (AFM) experiments by subjecting the cell model to compressive loads. The numerical role of the CSK components was corroborated with AFM force measurements on U2OS-osteosarcoma cells and NIH-3T3 fibroblasts exposed to different cytoskeleton-disrupting drugs. Computational simulation showed that actin cortex and microtubules are the major components targeted in resisting compression. This is a new numerical tool that explains the specific role of the cortex and overcomes the difficulty of isolating this component from other networks in vitro. This illustrates that a combination of cytoskeletal structures with their own properties is necessary for a complete description of cellular mechanics.
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影响因子：
3.4
作者：
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