Increased concentration of an indigenous proviral mouse mammary tumor virus long terminal repeat-containing transcript is associated with neoplastic transformation of mammary epithelium in C3H/Sm mice
Increased concentration of an indigenous proviral mouse mammary tumor virus long terminal repeat-containing transcript is associated with neoplastic transformation of mammary epithelium in C3H/Sm mice
复制标题
本地原病毒小鼠乳腺肿瘤病毒长末端重复转录物浓度的增加与 C3H/Sm 小鼠乳腺上皮的肿瘤转化有关
作者：
D. Graham;D. Medina;G. Smith
在 MMTV 阴性、C3H/Sm 小鼠乳腺肿瘤的 RNA 点印迹中发现小鼠乳腺肿瘤病毒 (MMTV) 前病毒转录物数量增加，这些肿瘤自发产生或由激素或化学致癌物或两者诱导。用长末端重复 (LTR) 探针探测的其他点印迹显示，这些肿瘤 RNA 中含有 LTR (MMTV) 的转录物不成比例地出现。使用 MMTV 基因组的不同片段与 Northern 印迹进行连续杂交，以确定转化乳腺组织中与泌乳乳腺中的相比，MMTV 转录物的相对序列内容和大小。在许多检查的肿瘤 RNA 中，使用 env 探针检测到 4.4-kilobase env 和 8.1-kilobase 基因组 MMTV 转录物数量增加。含有肿瘤 RNA 的相同 Northern 印迹与 LTR 探针的杂交揭示了一个 2.2-kilobase 的转录物，该转录物在化学诱导的、激素诱导的和自发性乳腺肿瘤的 RNA 中比来自哺乳期乳腺的 RNA 中突出。包含 LTR 的转录物与 env 或 gag-pol 探针不具有显着的同源性。这种独特的、转化增强的 2.2-kilobase 转录物除了 LTR 序列之外还可能包含小鼠细胞序列，或者它可能代表在四个 C3H/Sm MMTV 前病毒基因中的一个或多个的 LTR 开放阅读框中编码的非结构病毒蛋白的信息。
Increased amounts of mouse mammary tumor virus (MMTV) proviral transcripts were found in RNA dot blots from MMTV-negative, C3H/Sm mouse mammary tumors which arose spontaneously or were induced by hormonal or chemical carcinogens or both. Other dot blots probed with a long terminal repeat (LTR) probe showed that LTR (MMTV)-containing transcripts were disproportionately represented in these tumor RNAs. Different segments of the MMTV genome were used in sequential hybridizations to Northern blots to determine relative sequence content and size of MMTV transcripts in transformed mammary tissues, as compared with those in lactating mammary glands. Increased amounts of 4.4-kilobase env and 8.1-kilobase genomic MMTV transcripts were detected with an env probe in many of the tumor RNAs examined. Hybridization of the same Northern blots containing tumor RNAs with an LTR probe revealed a 2.2-kilobase transcript which was prominent in RNAs from chemically-induced, hormonally-induced, and spontaneous mammary tumors relative to those from lactating mammary glands. The LTR-containing transcript did not possess significant homology to either env or gag-pol probes. This distinctive, transformation-enhanced, 2.2-kilobase transcript may contain mouse cellular sequences in addition to LTR sequences or it may represent the message for a nonstructural viral protein encoded within the LTR open reading frame of one or more of the four C3H/Sm MMTV proviral genes.
小鼠胸腺瘤细胞系中小鼠乳腺肿瘤原病毒的去甲基化和表达。
DOI：
10.1073/pnas.80.1.110
发表时间：
1983
影响因子：
11.1
作者：
Mermod,JJ;Bourgeois,S;Defer,N;Crépin,M
通讯作者：
Crépin,M
化学致癌物-小鼠乳腺肿瘤病毒在细胞转化中的相互作用。
DOI：
10.1007/bf02617995
发表时间：
1983
期刊：
In vitro
影响因子：
--
作者：
Howard,DK;Schlom,J;Fisher,PB
通讯作者：
Fisher,PB
影响因子：
14.9
作者：
HOOPES, BC;MCCLURE, WR
通讯作者：
MCCLURE, WR