Computational reconstruction reveals a candidate magnetic biocompass to be likely irrelevant for magnetoreception.
Computational reconstruction reveals a candidate magnetic biocompass to be likely irrelevant for magnetoreception.
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DOI：
10.1038/s41598-017-13258-7
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发表时间：
2017-10-24
影响因子：
4.6
通讯作者：
Solov'yov IA
中科院分区：
文献类型：
--
作者：
Friis I;Sjulstok E;Solov'yov IA
鸟类利用地球的磁场在每年的迁徙旅行中导航。可能的机制，以解释生物物理学的罗盘感觉涉及电子转移内的感光蛋白隐色素。隐花色素的磁感受功能被认为是通过一个富含铁的聚合物复合物，耦合到多个隐花色素促进。本研究的目的是独立地重建该复合物，并描述其与果蝇隐花色素的相互作用。该聚合物复合物由ISCA 1蛋白单体与内部结合的铁硫簇组成，并同时结合10个隐花色素。通过分子动力学，我们分析了ISCA 1-隐花色素复合物的稳定性，并表征了单个隐花色素和ISCA 1之间结合位点的相互作用。发现隐花色素与ISCA 1聚合物的结合是不均匀的，并且结合亲和力取决于其沿着ISCA 1聚合物的位置。这一发现支持了这样的说法，即个别ISCA 1单体作为隐花色素的可能的细胞内相互作用伙伴，但提出的存在一个细长的ISCA 1聚合物与多个连接的隐花色素似乎是值得怀疑的。
Birds use the magnetic field of the Earth to navigate during their annual migratory travel. The possible mechanism to explain the biophysics of this compass sense involves electron transfers within the photoreceptive protein cryptochrome. The magnetoreceptive functioning of cryptochromes is supposedly facilitated through an iron rich polymer complex which couples to multiple cryptochromes. The present investigation aims to independently reconstruct this complex and describe its interaction with Drosophila melanogaster cryptochromes. The polymer complex consists of ISCA1 protein monomers with internally bound iron sulphur clusters and simultaneously binds ten cryptochromes. Through molecular dynamics we have analysed the stability of the ISCA1-cryptochrome complex and characterized the interaction at the binding sites between individual cryptochrome and ISCA1. It is found that the cryptochrome binding to the ISCA1 polymer is not uniform and that the binding affinity depends on its placement along the ISCA1 polymer. This finding supports the claim that the individual ISCA1 monomer acts as possible intracellular interaction partner of cryptochrome, but the proposed existence of an elongated ISCA1 polymer with multiple attached cryptochromes appears to be questionable.
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