M and N proteins of SARS coronavirus induce apoptosis in HPF cells.
M and N proteins of SARS coronavirus induce apoptosis in HPF cells.
复制标题
DOI：
10.1007/s10565-006-0077-1
复制
发表时间：
2006-09
影响因子：
6.1
通讯作者：
Peng JP
中科院分区：
文献类型：
--
作者：
Zhao G;Shi SQ;Yang Y;Peng JP
背景：SARS冠状病毒(SARS-CoV)诱导细胞凋亡，其结构蛋白可能在此过程中发挥作用。目的：探讨SARS冠状病毒结构蛋白M和N是否诱导细胞凋亡。研究设计：以人肺成纤维细胞为研究对象，将含有M基因或N基因的重组表达载体导入人肺成纤维细胞，TdT介导的缺口末端标记法(TUNEL)、Hoechst 33342染色及细胞形态观察。结果：在无血清条件下，pcDNA3.1-M转染组和N转染组细胞分别有16.34%和21.72%出现典型的细胞凋亡特征，与模型组(6.23%)相比差异有统计学意义(P<0.01)。此外，M蛋白和N蛋白共转染组的细胞死亡现象更为明显(约36.03%)。M转染组与共转染组比较差异有统计学意义(p<；0.01)，N转染组与共转染组比较差异有统计学意义(p<；0.01)。结论：SARS冠状病毒M蛋白和N蛋白均可诱导HPF细胞发生凋亡。M和N共转染可增强M或N单独诱导细胞凋亡的作用，这也提示SARS冠状病毒结构蛋白不仅在侵袭过程中可能发挥重要作用，而且在细胞的致病过程中也可能发挥重要作用。
Background: SARS-associated coronavirus (SARS-CoV) induced cell apoptosis and its structural proteins may play a role in this process. Objectives: To determine whether the structural proteins M and N of SARS-CoV induce apoptosis. Study design: We investigated human pulmonary fibroblast (HPF) cells, were transfected with plasmids containing the M or N gene, by TdT-mediated dUTP nick end labeling (TUNEL), Hoechst 33342 staining for nuclei, and observation of morphology. Results: We found that in the absence of serum about 16.34% of cells transfected by pcDNA3.1-M and 21.72% of N-transfected cells showed typical apoptotic characteristics, significantly different from mock-transfected cells (only 6.23%, p<0.01). Furthermore, the cells that were co-transfected with M and N proteins showed more obvious phenomena of cell death (about 36.03%). There was a statistical significance between M-transfected cells and co-transfected cells (p<0.01), and a remarkable difference between N-transfected cells and co-transfected cells (p<0.01). Conclusions: The results show that M and N proteins of SARS-CoV can induce apoptosis of HPF cells. Co-transfection of M and N enhances the induction of apoptosis by M or N alone, which also suggests that the structural proteins of SARS-CoV may play an important role not only in the process of invasion but also in the pathogenetic process in cells.
登录
查看更多内容
SARS-COV 3B蛋白在转染的细胞中诱导的G0/G1停滞和凋亡。
影响因子：
4.8
作者：
Yuan X;Shan Y;Zhao Z;Chen J;Cong Y
通讯作者：
Cong Y
影响因子：
3.8
作者：
Law, PTW;Wong, CH;Tsui, SKW
通讯作者：
Tsui, SKW
影响因子：
--
作者：
Gao, H;Peng, JP;Zhang, ZB
通讯作者：
Zhang, ZB
影响因子：
4.1
作者：
Yang, Y;Xiong, ZY;Yang, XF
通讯作者：
Yang, XF
影响因子：
4.4
作者：
Jiang, ZJ;Zhang, Y;Yu, ACH
通讯作者：
Yu, ACH