Effects of vaccine route and dosage on protection from rabies after intracerebral challenge in mice.
Effects of vaccine route and dosage on protection from rabies after intracerebral challenge in mice.
复制标题
疫苗途径和剂量对小鼠脑内攻击后狂犬病保护的影响。
DOI：
10.2460/ajvr.2003.64.491
复制
发表时间：
2003
影响因子：
1
通讯作者：
G. Baer
中科院分区：
文献类型：
--
作者：
P. Wunderli;D. W. Dreesen;T. Miller;G. Baer
客观化
目的探讨3种市售狂犬病疫苗不同给药途径和不同剂量对固定株狂犬病病毒脑内注射攻击小鼠血清抗体应答和保护作用的影响。
动物
2213只小鼠。
程序
灭活的佐剂狂犬病疫苗通过IP、IM和SC途径分别以2、1或0(对照)剂量注射给小鼠，并用固定株狂犬病病毒在小鼠脑内攻击。
结果
疫苗接种途径和剂量显著影响血清抗体反应和对狂犬病病毒攻击的保护，与疫苗株来源和小鼠株无关，尽管小鼠年龄显著影响结果。长期挑战研究表明，免疫小鼠的IM疫苗产生了与IP疫苗相当的最高血清中和抗体反应和保护水平。即使是多次注射SC(一种用于狗的疫苗接种途径)也会导致小鼠血清抗狂犬病中和抗体反应差，而且远远不如其他途径的保护作用。
结论与临床相关性
研究结果表明，目前在小鼠身上进行的狂犬病疫苗效力测试可能会通过使用1剂疫苗、IM途径和延迟挑战时间来改进。这些修改将更紧密地模拟目标物种的疫苗接种实践，并产生关于疫苗初级免疫原性的更准确信息。
OBJECTIVE
To evaluate the effect of various routes of administration and number of doses of 3 commercially produced rabies vaccines on serum antibody responses and protection in mice challenged by intracerebral injection with fixed-strain rabies virus.
ANIMALS
2,213 mice.
PROCEDURE
Inactivated, adjuvanted rabies vaccines were administered to mice in either 2, 1, or 0 (control) doses via IP, IM, and SC routes, and mice were challenged intracerebrally with fixed-strain rabies virus.
RESULTS
Vaccination route and dose number significantly influenced serum antibody responses and protection from rabies virus challenge, independent of vaccine strain origin and mouse strain, although mouse age significantly affected results. Extended challenge studies revealed that IM vaccination of mice resulted in the highest serum neutralizing antibody responses and protection levels equivalent to IP vaccination. Even multiple doses administered SC (a vaccination route used in dogs) resulted in poor serum anti-rabies neutralizing antibody responses in mice and were far less protective than other routes.
CONCLUSIONS AND CLINICAL RELEVANCE
Findings suggest possible improvements for the current rabies vaccine potency test in mice by using 1 dose, the IM route, and a delayed time of challenge. These modifications would more closely model vaccination practices in target species and yield more accurate information regarding primary immunogenicity of a vaccine.
通过免疫细胞的过继转移从实验性狂犬病中恢复。
DOI：
10.1099/0022-1317-56-1-25
发表时间：
1981
期刊：
The Journal of general virology
影响因子：
--
作者：
Prabhakar,BS;Fischman,HR;Nathanson,N
通讯作者：
Nathanson,N
DOI：
10.1073/pnas.84.24.9165
发表时间：
1987-12-01
影响因子：
11.1
作者：
DIETZSCHOLD, B;WANG, HH;KOPROWSKI, H
通讯作者：
KOPROWSKI, H