Degradation in the dentin-composite interface subjected to multi-species biofilm challenges.
Degradation in the dentin-composite interface subjected to multi-species biofilm challenges.
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DOI：
10.1016/j.actbio.2013.08.034
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发表时间：
2014-01
影响因子：
9.7
通讯作者：
Fok, A.
中科院分区：
文献类型：
--
作者：
Li, Y.;Carrera, C.;Chen, R.;Li, J.;Lenton, P.;Rudney, J. D.;Jones, R. S.;Aparicio, C.;Fok, A.
口腔生物膜可降解牙科树脂基复合修复体中的成分，从而影响边缘完整性并导致继发性龋病。在这项研究中，我们研究了多种口腔生物膜对牙本质-复合体界面机械完整性的影响。虽然大多数研究使用单一物种生物膜，但我们使用了更现实、更多样化的生物膜模型，直接从有早期儿童龋齿病史的捐赠者那里收集菌斑。用Z100™或Filtek™LS(3M ESPE)填充牛切牙根部制作牙本质复合盘。这些圆盘在成对的CDC生物膜反应器中孵化72小时，使用之前公布的方案。其中一个反应堆注入了蔗糖，另一个则没有。无菌纯唾液对照组用蔗糖脉动。在径向压缩条件下对盘片进行断裂，以评估其界面结合强度。使用数字图像相关(DIC)技术绘制磁盘表面变形图，以确定骨折来源。用扫描电子显微镜/能谱仪对断口进行了分析，以评估脱矿和界面退化情况。在蔗糖脉冲生物膜下，牙本质脱矿更严重，因为在脉冲过程中，pH降至5.5以下，LS和Z100样本遭受相似程度的表面矿物质丢失。蔗糖脉冲作用下的生物膜生长也会导致复合牙本质界面的优先降解，这取决于所使用的复合/粘结系统。具体而言，Z100试件表现出更大的粘结强度降低和更频繁的粘结层粘结破坏。这是由于Z100粘结剂对牙本质的覆盖率较低，可能导致更高水平的化学和酶降解。结果表明，牙本质脱矿以外的其他因素也是导致界面退化的原因。因此，我们开发了一个具有临床意义的体外生物膜模型，该模型将使我们能够有效地评估牙本质-复合体界面在多物种生物膜挑战下的退化。
Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries. In this study, we investigated the mechanical integrity of the dentin-composite interface challenged with multi-species oral biofilms. While most studies used single-species biofilms, we used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries. Dentin–composite disks were made using bovine incisor roots filled with Z100™ or Filtek™ LS (3M ESPE). The disks were incubated for 72hr in paired CDC biofilm reactors, using a previously published protocol. One reactor was pulsed with sucrose, and the other was not. A sterile saliva-only control group was run with sucrose pulsing. The disks were fractured under diametral compression to evaluate their interfacial bond strength. Surface deformation of the disks was mapped using digital image correlation (DIC) to ascertain fracture origin. Fracture surfaces were examined using SEM/EDS to assess demineralization and interfacial degradation. Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped below 5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss. Biofilm growth with sucrose pulsing also caused preferential degradation of the composite-dentin interface, depending on the composite/adhesive system used. Specifically, Z100 specimens showed greater bond strength reduction and more frequent cohesive failure in the adhesive layer. This was attributed to the inferior dentin coverage by Z100 adhesive which possibly led to a higher level of chemical and enzymatic degradation. The results suggested that factors other than dentin demineralization were also responsible for interfacial degradation. We have thus developed a clinically relevant in vitro biofilm model which would allow us to effectively assess the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
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影响因子：
14
作者：
Ito, S;Hashimoto, M;Pashley, DH
通讯作者：
Pashley, DH
影响因子：
4.4
作者：
Burke, FJT;Wilson, NHF;Mjör, IA
通讯作者：
Mjör, IA
影响因子：
3.2
作者：
Kreth, Jens;Zhang, Yongshu;Herzberg, Mark C.
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Herzberg, Mark C.
影响因子：
4.6
作者：
Manhart, J;Neuerer, P;Hickel, R
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Hickel, R
影响因子：
4.2
作者：
Cury, JA;Rebelo, MAB;Tabchoury, CPM
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Tabchoury, CPM