Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
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DOI：
10.1084/jem.194.9.1375
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发表时间：
2001-11-05
期刊：
影响因子：
--
通讯作者：
McEvoy LM
中科院分区：
文献类型：
--
作者：
Janatpour MJ;Hudak S;Sathe M;Sedgwick JD;McEvoy LM
从血液中募集的单核细胞是免疫反应性质的关键贡献者。虽然单核细胞募集在免疫病理的一个子集中已经得到了很好的研究，并且很大程度上归因于趋化因子单核细胞趋化蛋白(MCP)-1，但介导这种募集到其他炎症部位的机制仍然难以捉摸。本研究表明，局部炎症导致单核细胞与滤泡周围高内皮小静脉（HEVs）的结合增加，这些小静脉是引流局部炎症部位的淋巴结。定量PCR分析显示炎症淋巴结中许多趋化因子上调，包括MCP-1和MIG。hev未表达可检测水平的MCP-1；然而，野生型（而非肿瘤坏死因子[TNF]缺失小鼠）炎症淋巴结中的hev亚群表达MIG，并且该hev亚群优先支持单核细胞结合。在一小部分外周血单核细胞和相当比例的募集单核细胞中检测到MIG受体CXCR3的表达。最重要的是，在体内和体外实验中，中和的抗mig抗体阻断了单核细胞与炎症淋巴结hev的结合。总之，这些结果表明，淋巴结微环境可以以tnf依赖的方式决定HEV亚群上表达的分子的性质，并且HEV引起的炎症诱导的MIG表达可以介导单核细胞募集。
Monocytes recruited from the blood are key contributors to the nature of an immune response. While monocyte recruitment in a subset of immunopathologies has been well studied and largely attributed to the chemokine monocyte chemoattractant protein (MCP)-1, mechanisms mediating such recruitment to other sites of inflammation remain elusive. Here, we showed that localized inflammation resulted in an increased binding of monocytes to perifollicular high endothelial venules (HEVs) of lymph nodes draining a local inflammatory site. Quantitative PCR analyses revealed the upregulation of many chemokines in the inflamed lymph node, including MCP-1 and MIG. HEVs did not express detectable levels of MCP-1; however, a subset of HEVs in inflamed lymph nodes in wild-type (but not tumor necrosis factor [TNF] null mice) expressed MIG and this subset of HEVs preferentially supported monocyte binding. Expression of CXCR3, the receptor for MIG, was detected on a small subset of peripheral blood monocytes and on a significant percentage of recruited monocytes. Most importantly, in both ex vivo and in vivo assays, neutralizing anti-MIG antibodies blocked monocyte binding to inflamed lymph node HEVs. Together, these results suggest that the lymph node microenvironment can dictate the nature of molecules expressed on HEV subsets in a TNF-dependent fashion and that inflammation-induced MIG expression by HEVs can mediate monocyte recruitment.
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影响因子：
4.4
作者：
Sallusto, Federica;Lanzavecchia, Antonio
通讯作者：
Lanzavecchia, Antonio
影响因子：
56.9
作者：
Butcher, EC;Picker, LJ
通讯作者：
Picker, LJ
影响因子：
1.7
作者：
Rayner, K;Van Eersel, S;Reape, TJ
通讯作者：
Reape, TJ
DOI：
10.1084/jem.144.3.828
发表时间：
1976-09-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Stamper HB Jr;Woodruff JJ
通讯作者：
Woodruff JJ
影响因子：
5.4
作者：
UGUCCIONI, M;DAPUZZO, M;BAGGIOLINI, M
通讯作者：
BAGGIOLINI, M