Nidogen and heparan sulfate proteoglycan: detection of newly isolated basement membrane components in normal and epidermolysis bullosa skin.
Nidogen and heparan sulfate proteoglycan: detection of newly isolated basement membrane components in normal and epidermolysis bullosa skin.
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Nidogen 和硫酸乙酰肝素蛋白多糖：正常和大疱性表皮松解皮肤中新分离的基底膜成分的检测。
DOI：
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发表时间：
1987
影响因子：
6.5
通讯作者：
S. Katz
中科院分区：
文献类型：
--
作者：
S. Caughman;T. Krieg;R. Timpl;H. Hintner;S. Katz
表皮基底膜区包括各种生化成分，其中一些可能受到影响或参与某些形式的机械大疱性疾病。最近，巢蛋白和低密度形式的硫酸乙酰肝素蛋白聚糖-两个普遍存在的，非胶原成分的基底膜-被分离和鉴定，并制备亲和纯化的抗体，每个组件。这些抗体用于研究两种抗原在正常和患病的人皮肤中的分布。通过免疫荧光法，巢蛋白和硫酸乙酰肝素蛋白聚糖均沿正常人皮肤的真皮-表皮交界处的基底膜、附件结构和血管呈沿着线性分布。在正常皮肤的抽吸诱导的水疱上，在水疱的基部发现了两种抗原，这表明每种抗原都在透明板内或下方。通过间接免疫电镜观察，这两种抗原在超微结构上均位于致密板内。巢蛋白和硫酸乙酰肝素蛋白多糖的染色模式进行了检查，在11例交界性，显性营养不良，或隐性营养不良性大疱性表皮，并被发现是从正常皮肤中观察到的模式没有不同。
The epidermal basement membrane zone comprises various biochemical constituents, some of which may be affected or involved in certain forms of mechanobullous diseases. Recently, nidogen and a low density form of heparan sulfate proteoglycan--two ubiquitous, noncollagenous components of basement membranes--were isolated and characterized, and affinity-purified antibodies to each component were prepared. These antibodies were used to study the distribution of both antigens in normal and diseased human skin. By immunofluorescence, both nidogen and heparan sulfate proteoglycan were linearly distributed along the basement membrane of the dermal-epidermal junction, adnexal structures, and blood vessels of normal human skin. On suction-induced blisters of normal skin, both antigens were found at the base of the blister, indicating that each was within or below the lamina lucida. By indirect immunoelectron microscopy, both antigens were ultrastructurally located within the lamina densa. The staining patterns for nidogen and heparan sulfate proteoglycan were examined in 11 patients with either junctional, dominant dystrophic, or recessive dystrophic epidermolysis bullosa, and were found to be not different from the patterns observed in normal skin.
DOI：
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发表时间：
1981-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Carlin;R. Jaffe;B. Bender;A. Chung
通讯作者：
B. Carlin;R. Jaffe;B. Bender;A. Chung
影响因子：
--
作者：
Fine,JD;Gay,S
通讯作者：
Gay,S
锚定原纤维的单克隆抗体用于诊断大疱性表皮松解症。
DOI：
10.1111/1523-1747.ep12522669
发表时间：
1983
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Goldsmith,LA;Briggaman,RA
通讯作者：
Briggaman,RA