Bioinformatics analysis and construction of phylogenetic tree of aquaporins from Echinococcus granulosus
Bioinformatics analysis and construction of phylogenetic tree of aquaporins from Echinococcus granulosus
复制标题
细粒棘球绦虫水通道蛋白生物信息学分析及系统发育树构建
DOI：
10.1007/s00436-016-5114-2
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发表时间：
2016-05
影响因子：
2
通讯作者：
Ye Bin
中科院分区：
文献类型：
--
作者：
Wang Fen;Ye Bin
由细粒棘球蚴（Echinococcus granulosus，Eg）的裂头蚴幼虫引起的囊型棘球蚴病是一种慢性、世界性、严重的人畜共患寄生虫病。囊状棘球蚴病的治疗仍然很困难，因为手术不能满足所有患者的需要，药物可能导致严重的不良事件以及耐药性。筛选与抗包虫病药物相互作用的靶蛋白是当前研究的迫切需要。本文对该基因的序列和结构特征进行了分析，并利用生物信息学方法构建了系统发育树。在所有9个EgAQP中预测MIP家族特征和蛋白激酶C磷酸化位点。EgAQP的二级结构主要为α-螺旋和无规卷曲。EgAQP的跨膜区数目为3 ~ 6个，表明EgAQP含有多个疏水区。相邻连接树显示EgAQP分为两个分支，7个EgAQP与人的AQP 1（严格型水通道蛋白）形成一个分支，另外2个EgAQP与人的AQP 9（水甘油通道蛋白）形成一个分支。不幸的是，EgAQP的同源性建模被中止。这些结果为进一步了解和研究E.颗粒状。
Cyst echinococcosis caused by the matacestodal larvae of Echinococcus granulosus (Eg), is a chronic, worldwide, and severe zoonotic parasitosis. The treatment of cyst echinococcosis is still difficult since surgery cannot fit the needs of all patients, and drugs can lead to serious adverse events as well as resistance. The screen of target proteins interacted with new anti-hydatidosis drugs is urgently needed to meet the prevailing challenges. Here, we analyzed the sequences and structure properties, and constructed a phylogenetic tree by bioinformatics methods. The MIP family signature and Protein kinase C phosphorylation sites were predicted in all nine EgAQPs. α-helix and random coil were the main secondary structures of EgAQPs. The numbers of transmembrane regions were three to six, which indicated that EgAQPs contained multiple hydrophobic regions. A neighbor-joining tree indicated that EgAQPs were divided into two branches, seven EgAQPs formed a clade with AQP1 from human, a "strict" aquaporins, other two EgAQPs formed a clade with AQP9 from human, an aquaglyceroporins. Unfortunately, homology modeling of EgAQPs was aborted. These results provide a foundation for understanding and researches of the biological function of E. granulosus.
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DOI：
10.1016/j.bbagen.2013.10.007
发表时间：
2014-05
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
K. Tani;Y. Fujiyoshi
通讯作者：
K. Tani;Y. Fujiyoshi
影响因子：
3.4
作者：
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DOI：
10.1051/parasite/2014073
发表时间：
2014
期刊：
Parasite (Paris, France)
影响因子：
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作者：
Hemphill A;Stadelmann B;Rufener R;Spiliotis M;Boubaker G;Müller J;Müller N;Gorgas D;Gottstein B
通讯作者：
Gottstein B
DOI：
10.4238/2014.december.18.9
发表时间：
2014-12
期刊：
Genetics and molecular research : GMR
影响因子：
--
作者：
N. Z. Zhang;S. Y. Huang;D. Zhou;Y. Xu;J. J. He-J.;X. Zhu
通讯作者：
N. Z. Zhang;S. Y. Huang;D. Zhou;Y. Xu;J. J. He-J.;X. Zhu
影响因子：
4.8
作者：
Hansen, M;Kun, JFJ;Beitz, E
通讯作者：
Beitz, E