Electrophysiological properties of morphologically-identified medial vestibular nucleus neurons projecting to the abducens nucleus in the chick embryo.
Electrophysiological properties of morphologically-identified medial vestibular nucleus neurons projecting to the abducens nucleus in the chick embryo.
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DOI：
10.1016/j.neuroscience.2010.10.038
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发表时间：
2011-01-13
期刊：
影响因子：
3.3
通讯作者：
Peusner KD
中科院分区：
文献类型：
--
作者：
Gottesman-Davis A;Shao M;Hirsch JC;Peusner KD
内侧前庭核（MVN）的神经元显示出广泛的轴突投射通路、内在的放电特性和对头部运动的反应。为了确定参与前庭反射（VOR）的MVN神经元是否具有与其输出通路相关的独特电生理特性，设计了一种新的制备方法，使用含有鸡MVN和外展核的横向脑切片。将生物细胞素Alexa Fluor细胞外注射到外展核，使轴突投射到同侧（MVN/ABi）和对侧（MVN/ABc）外展核的MVN神经元被选择性标记。通过全细胞膜片钳记录，研究形态学鉴定的MVN/AB神经元的主动和被动膜特性、钠电导和自发突触事件，并将其与轴突无法追踪的MVN/n神经元进行比较。MVN/AB神经元主要位于MVN腹外侧的吻侧，主要具有直径为20 ~ 25 μm的星状细胞体。与MVNn神经元相比，MVN/ABi和MVN/ABc神经元具有更低的输入电阻。与所有其他MVN神经元组相比，MVN/ABc神经元表现出独特的放电特性，包括a型样波形，静息膜电位沉默，去极化时不能重复放电。有趣的是，自发兴奋性和抑制性突触事件的频率在所有MVN神经元中都是相似的。然而，与MVN/n神经元相比，MVN/ABi神经元的微型抑制事件与自发抑制事件的比例显著低于MVN/n神经元，这表明MVN/ABi神经元在脑切片中保留了更多和/或更活跃的抑制性突触前神经元。此外，与MVN/n神经元相比，MVN/ABi神经元具有更慢的衰减时间和半宽的mEPSCs。总之，这些发现强调了以轴突投射路径区分的MVN神经元类别的电生理特性的多样性。这是首次在脑切片制备中对MVN/AB神经元的研究，支持了体外脑切片制备为研究前庭信号处理中的细胞和分子事件提供了有利的模型。
Neurons in the medial vestibular nucleus (MVN) show a wide range of axonal projection pathways, intrinsic firing properties, and responses to head movements. To determine whether MVN neurons participating in the vestibulocular reflexes (VOR) have distinctive electrophysiological properties related to their output pathways, a new preparation was devised using transverse brain slices containing the chicken MVN and abducens nucleus. Biocytin Alexa Fluor was injected extracellularly into the abducens nucleus so that MVN neurons whose axons projected to the ipsilateral (MVN/ABi) and contralateral (MVN/ABc) abducens nuclei were labeled selectively. Whole-cell, patch-clamp recordings were performed to study the active and passive membrane properties, sodium conductances, and spontaneous synaptic events in morphologically-identified MVN/AB neurons and compare them to MVN neurons whose axons could not be traced (MVN/n). Located primarily in the rostral half of the ventrolateral part of the MVN, MVN/AB neurons mainly have stellate cell bodies with diameters of 20-25 μm. Compared to MVNn neurons, MVN/ABi and MVN/ABc neurons had lower input resistances. Compared to all other MVN neuron groups studied, MVN/ABc neurons showed unique firing properties, including type A-like waveform, silence at resting membrane potential, and failure to fire repetitively on depolarization. It is interesting that the frequency of spontaneous excitatory and inhibitory synaptic events was similar for all the MVN neurons studied. However, the ratio for miniature to spontaneous inhibitory events was significantly lower for MVN/ABi neurons compared to MVN/n neurons, suggesting that MVN/ABi neurons retained a larger number and/or more active inhibitory presynaptic neurons within the brain slices. Also, MVN/ABi neurons had mEPSCs with slower decay time and half width compared to MVN/n neurons. Altogether, these findings underscore the diversity of electrophysiological properties of MVN neuron classes distinguished by axonal projection pathways. This represents the first study of MVN/AB neurons in brain slice preparations and supports the concept that the in vitro brain slice preparation provides an advantageous model to investigate the cellular and molecular events in vestibular signal processing.
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影响因子：
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