iTRAQ-based proteomic analysis of myofibrillar contents and relevant synthesis and proteolytic proteins in soleus muscle of hibernating Daurian ground squirrels (Spermophilus dauricus).
iTRAQ-based proteomic analysis of myofibrillar contents and relevant synthesis and proteolytic proteins in soleus muscle of hibernating Daurian ground squirrels (Spermophilus dauricus).
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基于 iTRAQ 的蛋白质组学分析，对冬眠达乌尔地松鼠 (Spermophilus dauricus) 的比目鱼肌中肌原纤维含量及相关合成和蛋白水解蛋白进行分析
DOI：
10.1186/s12953-016-0105-x
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发表时间：
2016
期刊：
影响因子：
2
通讯作者：
Gao YF
中科院分区：
文献类型：
--
作者：
Chang H;Jiang SF;Dang K;Wang HP;Xu SH;Gao YF
BackgroundDaurian ground squirrels (Spermophilus dauricus) deviate from significant increase of protein catabolism and loss of myofibrillar contents during long period of hibernation inactivity.MethodsHere we use iTRAQ based quantitative analysis to examine proteomic changes in the soleus of squirrels in pre-hibernation, hibernation and post-hibernation states. The total proteolysis rate of soleus was measured by the release of the essential amino acid tyrosine from isolated muscles. Immunofluorescent analysis was used to determine muscle fiber cross-sectional area. Western blot was used for the validation of the quantitative proteomic analysis.ResultsThe proteomic responses to hibernation had a 0.4- to 0.8-fold decrease in the myofibrillar contractile protein levels of myosin-3, myosin-13 and actin, but a 2.1-fold increase in myosin-2 compared to pre-hibernation group. Regulatory proteins such as troponin C and tropomodulin-1 were 1.4-fold up-regulated and 0.7-fold down-regulated, respectively, in hibernation compared to pre-hibernation group. Moreover, 10 proteins with proteolytic function in hibernation, which was less than 14 proteins in the post-hibernation group, were up-regulated relative to the pre-hibernation group. The total proteolysis rates of soleus in hibernation and post-hibernation groups were significantly inhibited as compared with pre-hibernation group.ConclusionThese findings suggest that the myofibrillar remodeling and partial suppression of myofibrillar proteolysis were likely responsible for preventing skeletal muscle atrophy during prolonged disuse in hibernation. This is the first study where the myofibrillar contents and relevant synthesis and proteolytic proteins in slow soleus was discussed based on proteomic investigation performed on wild Daurian ground squirrels. Our results lay the foundation for further research in preventing disuse-induced skeletal muscle atrophy in mammals.
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DOI：
10.1152/ajpregu.00371.2013
发表时间：
2014-04-01
影响因子：
2.8
作者：
Lawler, John M.;Kunst, Mary;Martinez, Daniel A.
通讯作者：
Martinez, Daniel A.
影响因子：
3.3
作者：
Long, Meng;Zhao, Juan;Li, Aihua
通讯作者：
Li, Aihua
DOI：
10.1111/febs.12554
发表时间：
2013-12
期刊：
The FEBS journal
影响因子：
--
作者：
Doig J;Griffiths LA;Peberdy D;Dharmasaroja P;Vera M;Davies FJ;Newbery HJ;Brownstein D;Abbott CM
通讯作者：
Abbott CM
DOI：
10.1097/00005768-199610000-00007
发表时间：
1996-10-01
期刊：
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
影响因子：
--
作者：
Baldwin, KM;White, TP;Snow, CM
通讯作者：
Snow, CM
影响因子：
3.5
作者：
Garton, F. C.;Seto, J. T.;North, K. N.
通讯作者：
North, K. N.